The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Interactions of Small Nuclear RNA's with Precursor Messenger RNA During in Vitro Splicing
Abstract
Precursor messenger RNA splicing requires multiple factors including U1, U2, U4, U5, and U6 small nuclear RNA′s. The crosslinking reagent psoralen was used to analyze the interactions of these RNA′s with an adenovirus precursor messenger RNA in HeLa nuclear extract. An endogenous U2-U4-U6 crosslinkable complex dissociated upon incubation with precursor messenger RNA. During splicing, U1, U2, U5, and U6 became crosslinked to precursor messenger RNA and U2, U5, and U6 became crosslinked to excised lariat intron. U2 also formed a doubly crosslinked complex with U6 and precursor messenger RNA. The U1, U5, and U6 crosslinks to the precursor messenger RNA mapped to intron sequences near the 5′ splice site, whereas the U2 crosslink mapped to the branch site. The kinetics of crosslink formation and disappearance delineates a temporal pathway for the action of small RNA′s in the spliceosome. Potential base pairing interactions between conserved sequences in the small nuclear RNA′s and precursor messenger RNA at the sites of crosslinking suggest that the 5′ splice site is defined in several steps prior to the first cleavage event.
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Cites
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Self-citationBiochemical Mechanisms of Constitutive and Regulated Pre-mRNA Splicing
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Cited by
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14
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A general two-metal-ion mechanism for catalytic RNA
Steitz, T. A., & Steitz, J. A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.14.6498 · Cited by 1,171
Self-citationRNA Splicing by the Spliceosome
Wilkinson, M. E., Charenton, C., & Nagai, K. (2020) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-091719-064225 · Cited by 718
Protein–protein interactions and 5'-splice-site recognition in mammalian mRNA precursors
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Metadata
- Authors
- Wassarman, D. A., & Steitz, J. A.
- Venue
- Science
- Published
- 1992-09-25
- Volume
- 257
- Issue
- 5078
- Pages
- 1918-1925
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1979455742
- Cited by
- 362
- References
- 58
- FWCI
- 15.40
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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