The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Direct Gene Transfer into Mouse Muscle in Vivo
Abstract
RNA and DNA expression vectors containing genes for chloramphenicol acetyltransferase, luciferase, and β-galactosidase were separately injected into mouse skeletal muscle in vivo. Protein expression was readily detected in all cases, and no special delivery system was required for these effects. The extent of expression from both the RNA and DNA constructs was comparable to that obtained from fibroblasts transfected in vitro under optimal conditions. In situ cytochemical staining for β-galactosidase activity was localized to muscle cells following injection of the β-galactosidase DNA vector. After injection of the DNA luciferase expression vector, luciferase activity was present in the muscle for at least 2 months.
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In the citing paper
“In 1990, Wolff et al. (5) demonstrated that direct injection (”
From Nonviral delivery of self-amplifying RNA vaccines (2012) · cites this paper as [5]
In the citing paper
“Following initial studies in the early 1990s demonstrating that exogenous mRNA can direct protein expression in vivo, mRNA has emerged as a promising drug platform technology in recent years (Wolff et al, 1990; Jirikowski et al, 1992).”
From mRNA mediates passive vaccination against infectious agents, toxins, and tumors (2017) · cites this paper as Wolff et al, 1990
In the citing paper
“In principle, the improvement of RNA kinetics by these modifications is not restricted to DCs but is similar in K562 cells and may also be of interest for other cellular settings, such as T cells, 43 B cells, 44 and muscle cells, 45 which are attractive host cells for the expression of IVT RNA-encoded proteins.”
From Modification of antigen-encoding RNA increases stability, translational efficacy, and T-cell stimulatory capacity of dendritic cells (2006) · cites this paper as [45]
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Flagged first, then by citations
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- FRANZINIARMSTRO.C, MYOLOGY: 125 (1986).
- MALONE, R.W., FOCUS 11: 61 (1989).
Cited by
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mRNA vaccines — a new era in vaccinology
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Lipid nanoparticles for mRNA delivery
Hou, X., Zaks, T., Langer, R., & Dong, Y. (2021) · Nature Reviews Materials · doi:10.1038/s41578-021-00358-0 · Cited by 4,076
Effect of Surface Properties on Nanoparticle–Cell Interactions
Verma, A., & Stellacci, F. (2009) · Small · doi:10.1002/smll.200901158 · Cited by 2,621
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Sahin, U., Karikó, K., & Türeci, Ö. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4278 · Cited by 2,474
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Metadata
- Authors
- Wolff, J. A., Malone, R. W., Williams, P., Chong, W., Acsadi, G., Jani, A., & Felgner, P. L.
- Affiliations
- University of Wisconsin–Madison (US)
- Vical (United States) (US)
- Departments of Pediatrics and Genetics, Waisman Center, University of Wisconsin, Madison, WI 53706.
- Venue
- Science
- Published
- 1990-03-23
- Volume
- 247
- Issue
- 4949
- Pages
- 1465-1468
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2014495927
- Cited by
- 4,173
- References
- 27
- Citations by year
- 2026: 96 · 2025: 151 · 2024: 186 · 2023: 201 · 2022: 188 · 2021: 187 · 2020: 81 · 2019: 74 · 2018: 62 · 2017: 66 · 2016: 56 · 2015: 77 · 2014: 68 · 2013: 93 · 2012: 90
- FWCI
- 26.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5k11hd000669-02)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 03352, HD00669-05, P30HD003352)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Health Sciences › Medicine › Emergency Medicine › Intramuscular injections and effects
- Keywords
- in vivo gene delivery, skeletal muscle, intramuscular injection, DNA vectors, chloramphenicol acetyltransferase, luciferase, beta-galactosidase, transgene expression, cytochemical staining, transfection
- MeSH terms
- Animals, Avian Sarcoma Viruses, Coleoptera, beta-Galactosidase, DNA, Escherichia coli, Genetic Vectors, Histocytochemistry, Luciferases, Muscles, RNA, Transfection, Chloramphenicol O-Acetyltransferase, Gene Expression, Mice
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