Banded Marker Chromosomes as Indicators of Intraspecies Cellular Contamination
Abstract
Chromosome banding revealed marked chromosomes characteristic of HeLa cells in cultures designated HEK, HEK/HRV, HBT-3, HBT-39B, MA160, and a strain of SA-4TxS-Husa(1). Ohter HeLa cell characteristics found were glucose-6-phosphate dehydrogense type A mobility and lack the Y chromosome. Conventional chromosome analysis and immunological and enzymatic technique serve to monitor species specificity and racial origin of the donor. Chromosome banding, however, can monitor intralinear karyotype peculiarity and its evolution during long-term cultivation.
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The problems with cell line misidentification have been known for decades, commencing with the controversies around HeLa cells in the 1960s.
“The problems with cell line misidentification [5] have been known for decades, commencing with the controversies around HeLa cells in the 1960s [6–10].”
From The ghosts of HeLa: How cell line misidentification contaminates the scientific literature (2017) · cites this paper as [8]
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- Authors
- Nelson-Rees, W. A., Flandermeyer, R. R., & Hawthorne, P. K.
- Affiliations
- Cell Culture Laboratory, University of California, School of Public Health, Naval Biomedical Research Laboratory, Oakland 94625
- University of California System (US)
- Venue
- Science
- Published
- 1974-06-07
- Volume
- 184
- Issue
- 4141
- Pages
- 1093-1096
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1970487871
- Cited by
- 210
- References
- 0
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 4 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 5 · 2017: 4 · 2015: 1 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 18.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genomic variations and chromosomal abnormalities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- HeLa cells, karyotype evolution, long-term cell culture, species specificity, human cell lines
- MeSH terms
- Cell Line, Chromosomes, HeLa Cells, Humans, Karyotyping, Sex Chromosomes
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