The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Genetic Analysis of Histone H4: Essential Role of Lysines Subject to Reversible Acetylation
Abstract
The nucleosome is the fundamental unit of assembly of the chromosome and reversible modifications of the histones have been suggested to be important in many aspects of nucleosome function. The structure-function relations of the amino-terminal domain of yeast histone H4 were examined by the creation of directed point mutations. The four lysines subject to reversible acetylation were essential for histone function as the substitution of arginine or asparagine at these four positions was lethal. No single lysine residue was completely essential since arginine substitutions at each position were viable, although several of these mutants were slower in completing DNA replication. The simultaneous substitution of glutamine for the four lysine residues was viable but conferred several phenotypes including mating sterility, slow progression through the G 2 /M period of the division cycle, and temperature-sensitive growth, as well as a prolonged period of DNA replication. These results provide genetic proof for the roles of the H4 amino-terminal domain lysines in gene expression, replication, and nuclear division.
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Cited by
Histone acetylation in chromatin structure and transcription
Grunstein, M. (1997) · Nature · doi:10.1038/38664 · Cited by 2,884
The molecular hallmarks of epigenetic control
Allis, C. D., & Jenuwein, T. (2016) · Nature Reviews Genetics · doi:10.1038/nrg.2016.59 · Cited by 2,809
A Mammalian Histone Deacetylase Related to the Yeast Transcriptional Regulator Rpd3p
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Histone and chromatin cross-talk
Fischle, W., Wang, Y., & Allis, C. D. (2003) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(03)00013-9 · Cited by 1,207
SIRT1 Modulation of the Acetylation Status, Cytosolic Localization, and Activity of LKB1
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Metadata
- Authors
- Megee, P. C., Morgan, B. A., Mittman, B. A., & Smith, M. M.
- Venue
- Science
- Published
- 1990-02-16
- Volume
- 247
- Issue
- 4944
- Pages
- 841-845
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1966792552
- Cited by
- 349
- References
- 32
- FWCI
- 8.64
- Type
- journal article
- Language
- en
- Open access
- closed
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- Megee, P. C., Morgan, B. A., Mittman, B. A., & Smith, M. M. (1990). Genetic Analysis of Histone H4: Essential Role of Lysines Subject to Reversible Acetylation. Science. https://doi.org/10.1126/science.2106160
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
