Cited by 1,829Histone Deacetylase Inhibitors ResearchGenomics and Chromatin DynamicsEpigenetics and DNA Methylation

A Mammalian Histone Deacetylase Related to the Yeast Transcriptional Regulator Rpd3p

Taunton, Jack, Hassig, Christian A., Schreiber, Stuart L.·1996·Science

Abstract

Trapoxin is a microbially derived cyclotetrapeptide that inhibits histone deacetylation in vivo and causes mammalian cells to arrest in the cell cycle. A trapoxin affinity matrix was used to isolate two nuclear proteins that copurified with histone deacetylase activity. Both proteins were identified by peptide microsequencing, and a complementary DNA encoding the histone deacetylase catalytic subunit (HD1) was cloned from a human Jurkat T cell library. As the predicted protein is very similar to the yeast transcriptional regulator Rpd3p, these results support a role for histone deacetylase as a key regulator of eukaryotic transcription.

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Crossref · 10.1126/science.272.5260.408

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Authors
Taunton, J., Hassig, C. A., & Schreiber, S. L.
Affiliations
Howard Hughes Medical Institute (US)
Corresponding author
Schreiber, Stuart L.
Venue
Science
Published
1996-04-19
Volume
272
Issue
5260
Pages
408-411
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1974112332
Cited by
1,829
References
20
Citations by year
2026: 31 · 2025: 31 · 2024: 33 · 2023: 52 · 2022: 36 · 2021: 48 · 2020: 54 · 2019: 47 · 2018: 64 · 2017: 45 · 2016: 46 · 2015: 56 · 2014: 44 · 2013: 49 · 2012: 61
FWCI
36.98
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Histone Deacetylase Inhibitors Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
Keywords
histone deacetylase, cell cycle arrest, transcriptional regulation, yeast homolog, nuclear proteins, Jurkat cells, protein purification
MeSH terms
Amino Acid Sequence, Animals, Anti-Bacterial Agents, Cattle, Cell Cycle, Cloning, Molecular, Enzyme Inhibitors, Fungal Proteins, Gene Expression Regulation, Histone Deacetylases, Humans, Hydroxamic Acids, Molecular Sequence Data, Molecular Weight, Peptides, Saccharomyces cerevisiae, T-Lymphocytes, Transcription Factors, Transcription, Genetic, Tumor Cells, Cultured, Saccharomyces cerevisiae Proteins, Histone Deacetylase Inhibitors

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