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Sequence-Specific Packaging of DNA in Human Sperm Chromatin
Abstract
The DNA in human sperm chromatin is packaged into nucleoprotamine (∼85%) and nucleohistone (∼15%). Whether these two chromatin fractions are sequence-specific subsets of the spermatozoon genome is the question addressed in this report. Sequence-specific packaging would suggest distinct structural and functional roles for the nucleohistone and nucleoprotamine in late spermatogenesis or early development or both. After removal of histones with 0.65 M NaCl, exposed DNA was cleaved with Bam HI restriction endonuclease and separated by centrifugation from insoluble nucleoprotamine. The DNA sequence distribution of nucleohistone DNA in the supernatant and nucleoprotamine DNA in the pellet was compared by cloning size-selected single-copy sequences and by using the derived clones as probes of nucleohistone DNA and nucleoprotamine DNA. Two clones derived from nucleohistone DNA preferentially hybridized to nucleohistone DNA, and two clones derived from nucleoprotamine DNA preferentially hybridized to nucleoprotamine DNA, which demonstrated the existence of sequence-specific nucleohistone and nucleoprotamine components within the human spermatozoon.
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Citations
Cited by
The protamine family of sperm nuclear proteins
Balhorn, R. (2007) · Genome Biology · doi:10.1186/gb-2007-8-9-227 · Cited by 773
Self-citationRepressive and active histone methylation mark distinct promoters in human and mouse spermatozoa
Brykczynska, U., Hisano, M., Erkek, S., Ramos, L., Oakeley, E. J., Roloff, T. C., Beisel, C., Schübeler, D., Stadler, M. B., & Peters, A. H. F. M. (2010) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1821 · Cited by 680
Hong, L., Schroth, G. P., Matthews, H. R., Yau, P., & Bradbury, E. M. (1993) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54150-8 · Cited by 432
Self-citationImpact of obesity on male fertility, sperm function and molecular composition
Palmer, N. O., Bakos, H. W., Fullston, T., & Lane, M. (2012) · Spermatogenesis · doi:10.4161/spmg.21362 · Cited by 403
Function of sperm chromatin structural elements in fertilization and development
Ward, W. S. (2010) · Molecular Human Reproduction · doi:10.1093/molehr/gap080 · Cited by 395
Metadata
- Authors
- Gatewood, J. M., Cook, G. R., Balhorn, R., Bradbury, E. M., & Schmid, C. W.
- Venue
- Science
- Published
- 1987-05-22
- Volume
- 236
- Issue
- 4804
- Pages
- 962-964
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2053508758
- Cited by
- 383
- References
- 0
- FWCI
- 5.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- Gatewood, J. M., Cook, G. R., Balhorn, R., Bradbury, E. M., & Schmid, C. W. (1987). Sequence-Specific Packaging of DNA in Human Sperm Chromatin. Science. https://doi.org/10.1126/science.3576213
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