The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Genomic amplification with transcript sequencing
Abstract
A sequencing method called genomic amplification with transcript sequencing (GAWTS) is described that is based on amplification with the polymerase chain reaction (PCR). GAWTS bypasses cloning and increases the rate of sequence acquisition by at least fivefold. The method involves the attachment of a phage promoter onto at least one of the PCR primers. The segments amplified by PCR are transcribed to further increase the signal and to provide an abundance of single-stranded template for reverse transcriptase-mediated dideoxy sequencing. An end-labeled reverse transcriptase primer complementary to the desired sequence generates the additional specificity required to generate unambiguous sequence data. GAWTS can be performed on as little as a nanogram of genomic DNA. The rate of GAWTS can be increased by coamplification and cotranscription of multiple regions as illustrated by two regions of the factor IX gene. Since GAWTS lends itself well to automation, further increases in the rate of sequence acquisition can be expected.
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Cites
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Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1986) · Nature · doi:10.1038/324163a0 · Cited by 1,827
Fluorescence detection in automated DNA sequence analysis
Smith, L. M., Sanders, J. Z., Kaiser, R. J., Hughes, P., Dodd, C., Connell, C. R., Heiner, C., Kent, S. B. H., & Hood, L. E. (1986) · Nature · doi:10.1038/321674a0 · Cited by 1,714
Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences
Scharf, S. J., Horn, G. T., & Erlich, H. A. (1986) · Science · doi:10.1126/science.3461561 · Cited by 752
Complete nucleotide sequences of the gene for human factor IX (antihemophilic factor B)
Yoshitake, S., Schach, B. G., Foster, D. C., Davie, E. W., & Kurachi, K. (1985) · Biochemistry · doi:10.1021/bi00335a049 · Cited by 660
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Direct sequencing of enzymatically amplified human genomic DNA.
Engelke, D. R., Hoener, P. A., & Collins, F. S. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.2.544 · Cited by 137
Cited by
Post-transcriptional Regulation of Vascular Endothelial Growth Factor by Hypoxia
Levy, A. P., Levy, N. S., & Goldberg, M. A. (1996) · Journal of Biological Chemistry · doi:10.1074/jbc.271.5.2746 · Cited by 629
Schmidt, D., Goronzy, J. J., & Weyand, C. M. (1996) · Journal of Clinical Investigation · doi:10.1172/jci118638 · Cited by 482
The rapid detection of unknown mutations in nucleic acids
Grompe, M. (1993) · Nature Genetics · doi:10.1038/ng1093-111 · Cited by 310
The Design and Optimization of the PCR
Saiki, R. K. (1989) · PCR Technology · doi:10.1007/978-1-349-20235-5_1 · Cited by 258
Kusari, J., Verma, U. S., Buse, J. B., Henry, R. R., & Olefsky, J. M. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115437 · Cited by 90
Metadata
- Authors
- Stoflet, E., Koeberl, D., Sarkar, G., & Sommer, S.
- Venue
- Science
- Published
- 1988-01-29
- Volume
- 239
- Issue
- 4839
- Pages
- 491-494
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2074747188
- Cited by
- 320
- References
- 8
- FWCI
- 16.01
- Type
- journal article
- Language
- en
- Open access
- closed
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
