The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences
Abstract
A method is described for directly cloning enzymatically amplified segments of genomic DNA into an M13 vector for sequence analysis. A 110-base pair fragment of the human β-globin gene and a 242-base pair fragment of the human leukocyte antigen DQα locus were amplified by the polymerase chain reaction method, a procedure based on repeated cycles of denaturation, primer annealing, and extension by DNA polymerase I. Oligonucleotide primers with restriction endonuclease sites added to their 5′ ends were used to facilitate the cloning of the amplified DNA. The analysis of cloned products allowed the quantitative evaluation of the amplification method's specificity and fidelity. Given the low frequency of sequence errors observed, this approach promises to be a rapid method for obtaining reliable genomic sequences from nanogram amounts of DNA.
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Cites
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Self-citation
Cited by
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Self-citationWong, G. H. W., Elwell, J. H., Oberley, L. W., & Goeddel, D. V. (1989) · Cell · doi:10.1016/0092-8674(89)90944-6 · Cited by 802
Inference of haplotypes from PCR-amplified samples of diploid populations.
(1990) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040591 · Cited by 765
Kira, J., Koyanagi, Y., Yamada, T., Itoyama, Y., Goto, I., Yamamoto, N., Sasaki, H., & Sakaki, Y. (1991) · Annals of Neurology · doi:10.1002/ana.410290214 · Cited by 152
A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR
Sritharan, V., & Barker, R. H. (1991) · Molecular and Cellular Probes · doi:10.1016/s0890-8508(06)80011-3 · Cited by 127
Metadata
- Authors
- Scharf, S. J., Horn, G. T., & Erlich, H. A.
- Venue
- Science
- Published
- 1986-09-05
- Volume
- 233
- Issue
- 4768
- Pages
- 1076-1078
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2030903599
- Cited by
- 752
- References
- 1
- FWCI
- 22.66
- Type
- journal article
- Language
- en
- Open access
- closed
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- Scharf, S. J., Horn, G. T., & Erlich, H. A. (1986). Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences. Science. https://doi.org/10.1126/science.3461561
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
