AP1/ jun Function Is Differentially Induced in Promotion-Sensitive and Resistant JB6 Cells
Abstract
Tumor promoters may bring about events that lead to neoplastic transformation by inducing specific promotion-relevant effector genes. Functional activation of the transacting transcription factor AP-1 by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) may play an essential role in this process. Clonal genetic variants of mouse epidermal JB6 cells that are genetically susceptible (P + ) or resistant (P - ) to promotion of transformation by TPA were transfected with 3XTRE-CAT, a construct that has AP-1 cis-enhancer sequences attached to a reporter gene encoding chloramphenicol acetyltransferase (CAT). Transfected JB6 P + , but not P - variants, showed TPA-inducible CAT synthesis. Epidermal growth factor, another transformation promoter in JB6 cells, also caused P + specific induction of CAT gene expression. These results demonstrate an association between induced AP-1 function and sensitivity to promotion of neoplastic transformation.
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AP-1 is stimulated in promotion sensitive P+ JB6 cells but not in promotion resistant P- cells.
“Activation protein-1 (AP-1), a trans-acting transcription factor, was shown to be stimulated in the promotion sensitive (P+), but not in the promotion resistant (P-) cells [4,5].”
From Control Mechanisms of the Tumor Suppressor PDCD4: Expression and Functions (2019) · cites this paper as [5]
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- Authors
- Bernstein, L. R., & Colburn, N. H.
- Affiliations
- Johns Hopkins University (US)
- Frederick National Laboratory for Cancer Research (US)
- ORCID
- Bernstein, Lori R.
- Venue
- Science
- Published
- 1989-05-05
- Volume
- 244
- Issue
- 4904
- Pages
- 566-569
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1964303657
- Cited by
- 207
- References
- 34
- Citations by year
- 2025: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 6
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- AP-1 transcription factor, c-Jun, tumor promotion, oncogenic transformation, JB6 cells, phorbol myristate acetate, epidermal growth factor, chloramphenicol acetyltransferase reporter, epidermal cells
- MeSH terms
- Animals, Cell Line, Cell Transformation, Neoplastic, Cloning, Molecular, DNA-Binding Proteins, Epidermal Growth Factor, Epidermis, Gene Expression Regulation, Kinetics, Nucleic Acid Hybridization, Plasmids, Promoter Regions, Genetic, Proto-Oncogene Proteins, Tetradecanoylphorbol Acetate, Transcription Factors, Transfection, Genetic Variation, Chloramphenicol O-Acetyltransferase, Proto-Oncogene Proteins c-jun, Simplexvirus, Mice
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