Cited by 209Protein Kinase Regulation and GTPase SignalingCarcinogens and Genotoxicity AssessmentGenomics and Chromatin Dynamics

AP1/ jun Function Is Differentially Induced in Promotion-Sensitive and Resistant JB6 Cells

Bernstein, Lori R., Colburn, Nancy H.·1989·Science

Abstract

Tumor promoters may bring about events that lead to neoplastic transformation by inducing specific promotion-relevant effector genes. Functional activation of the transacting transcription factor AP-1 by the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) may play an essential role in this process. Clonal genetic variants of mouse epidermal JB6 cells that are genetically susceptible (P + ) or resistant (P - ) to promotion of transformation by TPA were transfected with 3XTRE-CAT, a construct that has AP-1 cis-enhancer sequences attached to a reporter gene encoding chloramphenicol acetyltransferase (CAT). Transfected JB6 P + , but not P - variants, showed TPA-inducible CAT synthesis. Epidermal growth factor, another transformation promoter in JB6 cells, also caused P + specific induction of CAT gene expression. These results demonstrate an association between induced AP-1 function and sensitivity to promotion of neoplastic transformation.

Integrity signals

Registry

Crossref · 10.1126/science.2541502

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • AP-1 is stimulated in promotion sensitive P+ JB6 cells but not in promotion resistant P- cells.

    “Activation protein-1 (AP-1), a trans-acting transcription factor, was shown to be stimulated in the promotion sensitive (P+), but not in the promotion resistant (P-) cells [4,5].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Bernstein, L. R., & Colburn, N. H.
Affiliations
  • Johns Hopkins University (US)
  • Frederick National Laboratory for Cancer Research (US)
Venue
Science
Published
1989-05-05
Volume
244
Issue
4904
Pages
566-569
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W1964303657
Cited by
207
References
34
Citations by year
2025: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2017: 4 · 2016: 2 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 6
FWCI
2.28
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
AP-1 transcription factor, c-Jun, tumor promotion, oncogenic transformation, JB6 cells, phorbol myristate acetate, epidermal growth factor, chloramphenicol acetyltransferase reporter, epidermal cells
MeSH terms
Animals, Cell Line, Cell Transformation, Neoplastic, Cloning, Molecular, DNA-Binding Proteins, Epidermal Growth Factor, Epidermis, Gene Expression Regulation, Kinetics, Nucleic Acid Hybridization, Plasmids, Promoter Regions, Genetic, Proto-Oncogene Proteins, Tetradecanoylphorbol Acetate, Transcription Factors, Transfection, Genetic Variation, Chloramphenicol O-Acetyltransferase, Proto-Oncogene Proteins c-jun, Simplexvirus, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review