The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Rational Design of Potent Antagonists to the Human Growth Hormone Receptor
Abstract
A hybrid receptor was constructed that contained the extracellular binding domain of the human growth hormone (hGH) receptor linked to the transmembrane and intracellular domains of the murine granulocyte colony-stimulating factor receptor. Addition of hGH to a myeloid leukemia cell line (FDC-P1) that expressed the hybrid receptor caused proliferation of these cells. The mechanism for signal transduction of the hybrid receptor required dimerization because monoclonal antibodies to the hGH receptor were agonists whereas their monovalent fragments were not. Receptor dimerization occurs sequentially—a receptor binds to site 1 on hGH, and then a second receptor molecule binds to site 2 on hGH. On the basis of this sequential mechanism, which may occur in many other cytokine receptors, inactive hGH analogs were designed that were potent antagonists to hGH-induced cell proliferation. Such antagonists could be useful for treating clinical conditions of hGH excess, such as acromegaly.
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Cites
Rapid and efficient site-specific mutagenesis without phenotypic selection.
Kunkel, T. A. (1985) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.82.2.488 · Cited by 7,206
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Kunkel, T. A., Roberts, J. D., & Zakour, R. A. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)54085-x · Cited by 5,261
Human Growth Hormone and Extracellular Domain of Its Receptor: Crystal Structure of the Complex
de Vos, A. M., Ultsch, M., & Kossiakoff, A. A. (1992) · Science · doi:10.1126/science.1549776 · Cited by 2,239
Structural design and molecular evolution of a cytokine receptor superfamily.
Bazan, J. F. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.18.6934 · Cited by 2,069
Growth hormone receptor and serum binding protein: purification, cloning and expression
Leung, D. W., Spencer, S. A., Cachianes, G., Hammonds, R. G., Collins, C., Henzel, W. J., Barnard, R., Waters, M. J., & Wood, W. I. (1987) · Nature · doi:10.1038/330537a0 · Cited by 1,522
High-Resolution Epitope Mapping of hGH-Receptor Interactions by Alanine-Scanning Mutagenesis
Cunningham, B. C., & Wells, J. A. (1989) · Science · doi:10.1126/science.2471267 · Cited by 1,367
Self-citationCunningham, B. C., Ultsch, M., de Vos, A. M., Mulkerrin, M. G., Clauser, K. R., & Wells, J. A. (1991) · Science · doi:10.1126/science.1948064 · Cited by 874
Self-citationA new cytokine receptor superfamily
Cosman, D., Lyman, S. D., Idzerda, R. L., Beckmann, M. P., Park, L. S., Goodwin, R. G., & March, C. J. (1990) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(90)90051-c · Cited by 476
Melmed, S. (1990) · New England Journal of Medicine · doi:10.1056/nejm199004053221405 · Cited by 438
Expression cloning of a receptor for murine granulocyte colony-stimulating factor
Fukunaga, R., Ishizaka-Ikeda, E., Seto, Y., & Nagata, S. (1990) · Cell · doi:10.1016/0092-8674(90)90814-u · Cited by 345
Self-citation
Cited by
Dimerization of cell surface receptors in signal transduction
Heldin, C.-H. (1995) · Cell · doi:10.1016/0092-8674(95)90404-2 · Cited by 1,634
Systemic Complications of Acromegaly: Epidemiology, Pathogenesis, and Management
Colao, A., Ferone, D., Marzullo, P., & Lombardi, G. (2004) · Endocrine Reviews · doi:10.1210/er.2002-0022 · Cited by 1,309
Sex Differences in the Expression of Hepatic Drug Metabolizing Enzymes
Waxman, D. J., & Holloway, M. G. (2009) · Molecular Pharmacology · doi:10.1124/mol.109.056705 · Cited by 815
Spivak-Kroizman, T., Lemmon, M. A., Dikic, I., Ladbury, J. E., Pinchasi, D., Huang, J., Jaye, M., Crumley, G., Schlessinger, J., & Lax, I. (1994) · Cell · doi:10.1016/0092-8674(94)90032-9 · Cited by 658
Binding in the growth hormone receptor complex.
Wells, J. A. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.1.1 · Cited by 438
Self-citation
Metadata
- Authors
- Fuh, G., Cunningham, B. C., Fukunaga, R., Nagata, S., Goeddel, D. V., & Wells, J. A.
- Venue
- Science
- Published
- 1992-06-19
- Volume
- 256
- Issue
- 5064
- Pages
- 1677-1680
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1988812517
- Cited by
- 631
- References
- 20
- FWCI
- 38.04
- Type
- journal article
- Language
- en
- Open access
- closed
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- Fuh, G., Cunningham, B. C., Fukunaga, R., Nagata, S., Goeddel, D. V., & Wells, J. A. (1992). Rational Design of Potent Antagonists to the Human Growth Hormone Receptor. Science. https://doi.org/10.1126/science.256.5064.1677
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
