The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization
Abstract
Vaccination with naked DNA elicits cellular and humoral immune responses that have a T helper cell type 1 bias. However, plasmid vectors expressing large amounts of gene product do not necessarily induce immune responses to the encoded antigens. Instead, the immunogenicity of plasmid DNA (pDNA) requires short immunostimulatory DNA sequences (ISS) that contain a CpG dinucleotide in a particular base context. Human monocytes transfected with pDNA or double-stranded oligonucleotides containing the ISS, but not those transfected with ISS-deficient pDNA or oligonucleotides, transcribed large amounts of interferon-α, interferon-β, and interleukin-12. Although ISS are necessary for gene vaccination, they down-regulate gene expression and thus may interfere with gene replacement therapy by inducing proinflammatory cytokines.
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Cites
CpG motifs in bacterial DNA trigger direct B-cell activation
Krieg, A. M., Yi, A.-K., Matson, S., Waldschmidt, T. J., Bishop, G. A., Teasdale, R., Koretzky, G. A., & Klinman, D. M. (1995) · Nature · doi:10.1038/374546a0 · Cited by 3,508
Cited by
DNA Vaccines: Immunology, Application, and Optimization
Gurunathan, S., Klinman, D. M., & Seder, R. A. (2000) · Annual Review of Immunology · doi:10.1146/annurev.immunol.18.1.927 · Cited by 1,207
Interleukin-12: A Cytokine at the Interface of Inflammation and Immunity
Trinchieri, G. (1998) · Advances in Immunology · doi:10.1016/s0065-2776(08)60387-9 · Cited by 760
Immunostimulatory DNA ameliorates experimental and spontaneous murine colitis
Rachmilewitz, D., Karmeli, F., Takabayashi, K., Hayashi, T., Leider-Trejo, L., Lee, J., Leoni, L. M., & Raz, E. (2002) · Gastroenterology · doi:10.1053/gast.2002.32994 · Cited by 384
Self-citationBacterial CpG DNA Activates Immune Cells to Signal Infectious Danger
Wagner, H. (1999) · Advances in Immunology · doi:10.1016/s0065-2776(08)60790-7 · Cited by 314
Elkins, K. L., Rhinehart-Jones, T. R., Stibitz, S., Conover, J. S., & Klinman, D. M. (1999) · The Journal of Immunology · doi:10.4049/jimmunol.162.4.2291 · Cited by 314
Metadata
- Authors
- Sato, Y., Roman, M., Tighe, H., Lee, D., Corr, M., Nguyen, M.-D., Silverman, G. J., Lotz, M., Carson, D. A., & Raz, E.
- Venue
- Science
- Published
- 1996-07-19
- Volume
- 273
- Issue
- 5273
- Pages
- 352-354
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2066833587
- Cited by
- 1,058
- References
- 1
- FWCI
- 40.20
- Type
- journal article
- Language
- en
- Open access
- closed
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- Sato, Y., Roman, M., Tighe, H., Lee, D., Corr, M., Nguyen, M.-D., Silverman, G. J., Lotz, M., Carson, D. A., & Raz, E. (1996). Immunostimulatory DNA Sequences Necessary for Effective Intradermal Gene Immunization. Science. https://doi.org/10.1126/science.273.5273.352
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