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Crystal Structure of a Group I Ribozyme Domain: Principles of RNA Packing
Abstract
Group I self-splicing introns catalyze their own excision from precursor RNAs by way of a two-step transesterification reaction. The catalytic core of these ribozymes is formed by two structural domains. The 2.8-angstrom crystal structure of one of these, the P4-P6 domain of the Tetrahymena thermophila intron, is described. In the 160-nucleotide domain, a sharp bend allows stacked helices of the conserved core to pack alongside helices of an adjacent region. Two specific long-range interactions clamp the two halves of the domain together: a two-Mg 2+ -coordinated adenosine-rich corkscrew plugs into the minor groove of a helix, and a GAAA hairpin loop binds to a conserved 11-nucleotide internal loop. Metal- and ribose-mediated backbone contacts further stabilize the close side-by-side helical packing. The structure indicates the extent of RNA packing required for the function of large ribozymes, the spliceosome, and the ribosome.
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Citations
Cited by
Mathews, D. H., Sabina, J., Zuker, M., & Turner, D. H. (1999) · Journal of Molecular Biology · doi:10.1006/jmbi.1999.2700 · Cited by 3,795
The non-Watson-Crick base pairs and their associated isostericity matrices
Leontis, N. B. (2002) · Nucleic Acids Research · doi:10.1093/nar/gkf481 · Cited by 796
Engineered pegRNAs improve prime editing efficiency
Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, A. V., An, M., Newby, G. A., Chen, J. C., Hsu, A., et al. (2022) · Nature Biotechnology · doi:10.1038/s41587-021-01039-7 · Cited by 727
The chemical repertoire of natural ribozymes
Doudna, J. A., & Cech, T. R. (2002) · Nature · doi:10.1038/418222a · Cited by 745
Self-citationDirected Evolution of Nucleic Acid Enzymes
Joyce, G. F. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073717 · Cited by 515
Metadata
- Authors
- Cate, J. H., Gooding, A. R., Podell, E., Zhou, K., Golden, B. L., Kundrot, C. E., Cech, T. R., & Doudna, J. A.
- Venue
- Science
- Published
- 1996-09-20
- Volume
- 273
- Issue
- 5282
- Pages
- 1678-1685
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2044247083
- Cited by
- 1,248
- References
- 0
- FWCI
- 35.01
- Type
- journal article
- Language
- en
- Open access
- closed
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- Cate, J. H., Gooding, A. R., Podell, E., Zhou, K., Golden, B. L., Kundrot, C. E., Cech, T. R., & Doudna, J. A. (1996). Crystal Structure of a Group I Ribozyme Domain: Principles of RNA Packing. Science. https://doi.org/10.1126/science.273.5282.1678
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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