The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
In Situ Visualization of DNA Double-Strand Break Repair in Human Fibroblasts
Abstract
A method was developed to examine DNA repair within the intact cell. Ultrasoft x-rays were used to induce DNA double-strand breaks (DSBs) in defined subnuclear volumes of human fibroblasts and DNA repair was visualized at those sites. The DSBs remained in a fixed position during the initial stages of DNA repair, and the DSB repair protein hMre11 migrated to the sites of damage within 30 minutes. In contrast, hRad51, a human RecA homolog, did not localize at sites of DNA damage, a finding consistent with the distinct roles of these proteins in DNA repair.
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“The Mre11/Rad50/Nbs1 (MRN) complex recognizes the resulting DSBs and facilitates ATM recruitment and activation [20–23].”
From ATR- and ATM-Mediated DNA Damage Response Is Dependent on Excision Repair Assembly during G1 but Not in S Phase of Cell Cycle (2016) · cites this paper as [22]
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Megabase Chromatin Domains Involved in DNA Double-Strand Breaks in Vivo
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Metadata
- Authors
- Nelms, B. E., Maser, R. S., MacKay, J. F., Lagally, M. G., & Petrini, J. H. J.
- Affiliations
- University of Wisconsin System (US)
- Corresponding author
- Nelms, Benjamin E.
- Maser, Richard S.
- Petrini, John H. J.
- ORCID
- Petrini, John H. J.
- Venue
- Science
- Published
- 1998-04-24
- Volume
- 280
- Issue
- 5363
- Pages
- 590-592
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2054889035
- Cited by
- 500
- References
- 38
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 6 · 2022: 3 · 2021: 3 · 2020: 10 · 2019: 3 · 2018: 11 · 2017: 7 · 2016: 13 · 2015: 5 · 2014: 21 · 2013: 16 · 2012: 13
- FWCI
- 12.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (T32GM008349, T32GM007133)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
- Keywords
- DNA double-strand break repair, human fibroblasts, in situ visualization, ultrasoft X-rays, RecA homolog, subnuclear localization, DNA damage response, protein localization
- MeSH terms
- Bromodeoxyuridine, Cell Line, Cell Nucleus, DNA, DNA Damage, DNA Repair, DNA-Binding Proteins, Fibroblasts, Fluorescent Antibody Technique, Humans, Fluorescein-5-isothiocyanate, Microscopy, Confocal, Rad51 Recombinase
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