Cited by 499DNA Repair MechanismsEffects of Radiation ExposureFungal and yeast genetics research

hMre11 and hRad50 Nuclear Foci Are Induced During the Normal Cellular Response to DNA Double-Strand Breaks †

Open access
Maser, Richard S., Monsen, Kirsten J., Nelms, Benjamin E., Petrini, John H. J.·1997·Molecular and Cellular Biology

Abstract

We previously identified a conserved multiprotein complex that includes hMre11 and hRad50. In this study, we used immunofluorescence to investigate the role of this complex in DNA double-strand break (DSB) repair. hMre11 and hRad50 form discrete nuclear foci in response to treatment with DSB-inducing agents but not in response to UV irradiation. hMre11 and hRad50 foci colocalize after treatment with ionizing radiation and are distinct from those of the DSB repair protein, hRad51. Our data indicate that an irradiated cell is competent to form either hMre11-hRad50 foci or hRad51 foci, but not both. The multiplicity of hMre11 and hRad50 foci is much higher in the DSB repair-deficient cell line 180BR than in repair-proficient cells. hMre11-hRad50 focus formation is markedly reduced in cells derived from ataxia-telangiectasia patients, whereas hRad51 focus formation is markedly increased. These experiments support genetic evidence from Saccharomyces cerevisiae indicating that Mre11-Rad50 have roles distinct from that of Rad51 in DSB repair. Further, these data indicate that hMre11-hRad50 foci form in response to DNA DSBs and are dependent upon a DNA damage-induced signaling pathway.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1128/mcb.17.10.6087

Cites

81 of 81 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Eight hours after DNA damage, a portion of cells contain prominent Mre11/Rad50/Nbs1 foci, and these foci are thought to be sites of remaining DNA damage.

    “Previous studies have demonstrated that 8 h after DNA damage, a portion of cells contain prominent Mre11/Rad50/Nbs1 foci, and these foci are thought to be sites of remaining DNA damage (8, 30).”

    From Mdm2 Promotes Genetic Instability and Transformation Independent of p53 (2008) · cites this paper as [30]

    The Results (page not available) report that more than 60% of irradiated cells had hMre11 and hRad50 foci at 8 hours. The paper does not establish Nbs1 as part of those foci, and its Conclusion (page not available) says that locating the foci at DNA break sites awaits further study. Thus, it supports the timing and presence of foci, but not the full claim about their composition and location at remaining damage. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 1

  1. Friedberg, E. C., G. C. Walker, and W. Siede. 1995. DNA repair and mu- tagenesis. ASM Press, Washington, D.C.

Cited by

Sorted by citations

Authors
Maser, R. S., Monsen, K. J., Nelms, B. E., & Petrini, J. H. J.
Affiliations
  • University of Wisconsin–Madison (US)
  • University of Wisconsin System (US)
Corresponding author
Petrini, John H. J.
Venue
Molecular and Cellular Biology
Published
1997-10
Volume
17
Issue
10
Pages
6087-6096
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W2131646714
Cited by
499
References
81
Citations by year
2026: 2 · 2025: 2 · 2024: 2 · 2023: 5 · 2022: 4 · 2021: 10 · 2020: 11 · 2019: 4 · 2018: 6 · 2017: 3 · 2016: 6 · 2015: 12 · 2014: 9 · 2013: 12 · 2012: 14
FWCI
9.46
Type
journal article
Language
en
Open access
Locations
  • Molecular and Cellular Biology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Howard Hughes Medical Institute
  • American Cancer Society
  • March of Dimes Foundation
  • University of Wisconsin-Madison
  • National Institutes of Health
  • National Institute of General Medical Sciences (T32GM008349, T32GM007133)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
Mre11-Rad50 complex, DNA double-strand break repair, nuclear foci, RAD51, ataxia telangiectasia, ionizing radiation, UV irradiation, DNA damage signaling
MeSH terms
Ataxia Telangiectasia, Cell Nucleus, Cells, Cultured, DNA Damage, DNA Repair, DNA-Binding Proteins, Endodeoxyribonucleases, Enzyme Inhibitors, Etoposide, Exodeoxyribonucleases, Fibroblasts, Fungal Proteins, Gamma Rays, Humans, Mutation, Proteins, Signal Transduction, G2 Phase, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Tumor Suppressor Proteins, Saccharomyces cerevisiae Proteins, Rad51 Recombinase, Topoisomerase II Inhibitors, Ataxia Telangiectasia Mutated Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review