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Chemical Amplification: Continuous-Flow PCR on a Chip
Abstract
A micromachined chemical amplifier was successfully used to perform the polymerase chain reaction (PCR) in continuous flow at high speed. The device is analogous to an electronic amplifier and relies on the movement of sample through thermostated temperature zones on a glass microchip. Input and output of material (DNA) is continuous, and amplification is independent of input concentration. A 20-cycle PCR amplification of a 176–base pair fragment from the DNA gyrase gene of Neisseria gonorrhoeae was performed at various flow rates, resulting in total reaction times of 90 seconds to 18.7 minutes.
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Citations
Cited by
Zhu, H., Zhang, H., Xu, Y., Laššáková, S., Korabečná, M., & Neužil, P. (2020) · BioTechniques · doi:10.2144/btn-2020-0057 · Cited by 380
(1983) · Environmental Science & Technology · doi:10.1021/es00115a738 · Cited by 328
Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis
Lagally, E. T., Emrich, C. A., & Mathies, R. A. (2001) · Lab on a Chip · doi:10.1039/b109031n · Cited by 284
Polymerase chain reaction in microfluidic devices
Ahrberg, C. D., Manz, A., & Chung, B. G. (2016) · Lab on a Chip · doi:10.1039/c6lc00984k · Cited by 283
Self-citationPreservation of key biomolecules in the fossil record: current knowledge and future challenges
Bada, J. L., Wang, X. S., & Hamilton, H. (1999) · Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rstb.1999.0361 · Cited by 147
Metadata
- Authors
- Kopp, M. U., Mello, A. J. D., & Manz, A.
- Venue
- Science
- Published
- 1998-05-15
- Volume
- 280
- Issue
- 5366
- Pages
- 1046-1048
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2074305200
- Cited by
- 1,212
- References
- 0
- FWCI
- 45.96
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kopp, M. U., Mello, A. J. D., & Manz, A. (1998). Chemical Amplification: Continuous-Flow PCR on a Chip. Science. https://doi.org/10.1126/science.280.5366.1046
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
