The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis
Abstract
A fully integrated genomic analysis microsystem including microfabricated heaters, temperature sensors, and PCR chambers directly connected to capillary electrophoretic separation channels has been constructed. Valves and hydrophobic vents provide controlled and sensorless sample positioning and immobilization into 200 nL PCR chambers. The use of microfabricated heating and temperature sensing elements improves the heating and cooling rates for the PCR reaction to 20 degree C s(-1). The amplified PCR product, labeled on-column with an intercalating fluorescent dye, is injected into the gel-filled capillary for electrophoretic analysis. Successful sex determination using a multiplex PCR reaction from human genomic DNA is demonstrated in less than 15 min. This device is an important step toward a microfabricated genomic microprocessor for use in forensics and point-of-care molecular medical diagnostics.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G., & Erlich, H. (1986) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1986.051.01.032 · Cited by 3,558
High-performance electrophoresis
Hjertén, S. (1985) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)95485-8 · Cited by 1,513
(1995) · Springer eBooks · doi:10.1007/978-94-011-0161-5 · Cited by 1,250
Chemical Amplification: Continuous-Flow PCR on a Chip
Kopp, M. U., Mello, A. J. D., & Manz, A. (1998) · Science · doi:10.1126/science.280.5366.1046 · Cited by 1,212
Woolley, A. T., Hadley, D., Landre, P., deMello, A. J., Mathies, R. A., & Northrup, M. A. (1996) · Analytical Chemistry · doi:10.1021/ac960718q · Cited by 730
Self-citationSingle-Molecule DNA Amplification and Analysis in an Integrated Microfluidic Device
Lagally, E. T., Medintz, I. L., & Mathies, R. A. (2001) · Analytical Chemistry · doi:10.1021/ac001026b · Cited by 430
Self-citationMicrochip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing
Waters, L. C., Jacobson, S. C., Kroutchinina, N., Khandurina, J., Foote, R. S., & Ramsey, J. M. (1998) · Analytical Chemistry · doi:10.1021/ac970642d · Cited by 405
Integrated Cell Isolation and Polymerase Chain Reaction Analysis Using Silicon Microfilter Chambers
Wilding, P., Kricka, L. J., Cheng, J., Hvichia, G., Shoffner, M. A., & Fortina, P. (1998) · Analytical Biochemistry · doi:10.1006/abio.1997.2530 · Cited by 276
A Miniature Analytical Instrument for Nucleic Acids Based on Micromachined Silicon Reaction Chambers
Northrup, M. A., Benett, B., Hadley, D. R., Landre, P., Lehew, S., Richards, J. M., & Stratton, P. (1998) · Analytical Chemistry · doi:10.1021/ac970486a · Cited by 266
Real-Time Microchip PCR for Detecting Single-Base Differences in Viral and Human DNA
Ibrahim, M. S., Lofts, R. S., Jahrling, P. B., Henchal, E. A., Weedn, V. W., Northrup, M. A., & Belgrader, P. (1998) · Analytical Chemistry · doi:10.1021/ac971091u · Cited by 152
Cited by
Microfluidic systems in proteomics
Lion, N., Rohner, T. C., Dayon, L., Arnaud, I. L., Damoc, E., Youhnovski, N., Wu, Z., Roussel, C., Josserand, J., Jensen, H., et al. (2003) · ELECTROPHORESIS · doi:10.1002/elps.200305629 · Cited by 262
Thompson, B. L., Ouyang, Y., Duarte, G. R. M., Carrilho, E., Krauss, S. T., & Landers, J. P. (2015) · Nature Protocols · doi:10.1038/nprot.2015.051 · Cited by 149
Metadata
- Authors
- Lagally, E. T., Emrich, C. A., & Mathies, R. A.
- Venue
- Lab on a Chip
- Publisher
- Royal Society of Chemistry (RSC)
- Published
- 2001
- Volume
- 1
- Issue
- 2
- Pages
- 102
- ISSN
- 1473-0189 (ISSN-L)
- 1473-0197
- OpenAlex
- W2144498825
- Cited by
- 284
- References
- 11
- FWCI
- 10.79
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis. https://app.cochat.ai/papers/10.1039/b109031n
- The paper
- Lagally, E. T., Emrich, C. A., & Mathies, R. A. (2001). Fully integrated PCR-capillary electrophoresis microsystem for DNA analysis. Lab on a Chip. https://doi.org/10.1039/b109031n
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
