Complete Structure of the 11-Subunit Bovine Mitochondrial Cytochrome bc 1 Complex
Abstract
Mitochondrial cytochrome bc1 complex performs two functions: It is a respiratory multienzyme complex and it recognizes a mitochondrial targeting presequence. Refined crystal structures of the 11-subunit bc1 complex from bovine heart reveal full views of this bifunctional enzyme. The "Rieske" iron-sulfur protein subunit shows significant conformational changes in different crystal forms, suggesting a new electron transport mechanism of the enzyme. The mitochondrial targeting presequence of the "Rieske" protein (subunit 9) is lodged between the two "core" subunits at the matrix side of the complex. These "core" subunits are related to the matrix processing peptidase, and the structure unveils how mitochondrial targeting presequences are recognized.
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RISP normally transfers electrons from ubiquinol to cytochrome c1 in complex III.
“First, we knocked down the mitochondrial RISP, which normally transfers electrons from ubiquinol to cytochrome c1 in complex III (18).”
From Mitochondrial Dysfunction Contributes to Oncogene-Induced Senescence (2009) · cites this paper as [18]
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- Authors
- Iwata, S., Lee, J. W., Okada, K., Lee, J. K., Iwata, M., Rasmussen, B., Link, T. A., Ramaswamy, S., & Jap, B. K.
- Affiliations
- Uppsala University (SE)
- Corresponding author
- Iwata, So
- Okada, Kengo
- Jap, Bing K.
- Venue
- Science
- Published
- 1998-07-03
- Volume
- 281
- Issue
- 5373
- Pages
- 64-71
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2170621128
- Cited by
- 1,253
- References
- 0
- Citations by year
- 2026: 16 · 2025: 27 · 2024: 21 · 2023: 22 · 2022: 35 · 2021: 49 · 2020: 27 · 2019: 26 · 2018: 33 · 2017: 31 · 2016: 35 · 2015: 37 · 2014: 39 · 2013: 34 · 2012: 56
- FWCI
- 74.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Materials Chemistry › Enzyme Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- cytochrome bc1 complex, mitochondrial respiratory chain, Rieske iron-sulfur protein, electron transport mechanism, conformational change, bovine heart mitochondria, crystal structure
- MeSH terms
- Amino Acid Sequence, Animals, Binding Sites, Cattle, Crystallization, Cytochrome b Group, Cytochromes c1, Electron Transport, Enzyme Inhibitors, Hydrogen Bonding, Hydroquinones, Intracellular Membranes, Iron-Sulfur Proteins, Methacrylates, Mitochondria, Heart, Models, Molecular, Molecular Sequence Data, Oxidation-Reduction, Protein Conformation, Thiazoles, Electron Transport Complex III, Protein Structure, Secondary, Crystallography, X-Ray
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