Cited by 119Cardiac Ischemia and ReperfusionMitochondrial Function and PathologyCell death mechanisms and regulation

Depletion of cardiolipin and cytochrome c during ischemia increases hydrogen peroxide production from the electron transport chain

Chen, Qun, Lesnefsky, Edward J.·2006·Free Radical Biology and Medicine

Abstract

Mitochondrial electron transport is a major source of reactive oxygen species (ROS) during cardiac ischemia and reperfusion. In the isolated rabbit heart, 30 and 45 min of ischemia decrease the contents of cardiolipin and cytochrome c in subsarcolemmal mitochondria (SSM) located beneath the plasma membrane. In contrast, interfibrillar mitochondria (IFM) in the interior of the myocyte do not sustain a decrease in cardiolipin. We proposed that the depletion of cardiolipin and the accompanying cytochrome c loss during ischemia were critical events that amplified ROS production by mitochondria. The total production of H2O2 was measured in submitochondrial particles (SMP) prepared from rabbit heart SSM and IFM after 0, 15, 30, and 45 min of ischemia. With NADH as substrate, total H2O2 production was increased only in SMP from SSM after 30 and 45 min ischemia, when ischemia decreased the content of cardiolipin and cytochrome c. In contrast, ischemia did not augment H2O2 generation in SMP from IFM with preserved cardiolipin and cytochrome c content. Thus, during the evolution of ischemic injury, H2O2 production from the electron transport chain increased after depletion of cardiolipin and the loss of cytochrome c.

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Crossref · 10.1016/j.freeradbiomed.2005.10.043

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Authors
Chen, Q., & Lesnefsky, E. J.
Affiliations
  • Xuzhou Medical College (CN)
  • Louis Stokes Cleveland VA Medical Center (US)
Corresponding author
Lesnefsky, Edward J.
Venue
Free Radical Biology and Medicine
Publisher
Elsevier BV
Published
2006-03
Volume
40
Issue
6
Pages
976-982
ISSN
0891-5849 (ISSN-L)
OpenAlex
W2092718544
Cited by
119
References
42
Citations by year
2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 5 · 2021: 3 · 2020: 6 · 2019: 2 · 2018: 6 · 2017: 6 · 2016: 6 · 2015: 5 · 2014: 9 · 2013: 11 · 2012: 6
FWCI
4.23
Type
journal article
Language
en
Open access
closed
Locations
  • Free Radical Biology and Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • U.S. Department of Veterans Affairs
  • American Heart Association
  • Office of Research and Development
  • National Institutes of Health
  • National Institute on Aging (1PO1 AG-15885, P01AG015885)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Keywords
cytochrome c, hydrogen peroxide production, mitochondrial electron transport chain, myocardial ischemia, reperfusion, reactive oxygen species, subsarcolemmal mitochondria, interfibrillar mitochondria, submitochondrial particles, NADH oxidation, rabbit heart
MeSH terms
Animals, Antimycin A, Cardiolipins, Electron Transport Complex IV, Electron Transport, Hydrogen Peroxide, Mitochondria, Heart, Rabbits, Rotenone, Myocardial Reperfusion Injury, Myocardial Ischemia, Sodium Azide, Cytochromes c

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