C 1 Transfer Enzymes and Coenzymes Linking Methylotrophic Bacteria and Methanogenic Archaea
Abstract
Methanogenic and sulfate-reducing Archaea are considered to have an energy metabolism involving C 1 transfer coenzymes and enzymes unique for this group of strictly anaerobic microorganisms. An aerobic methylotrophic bacterium, Methylobacterium extorquens AM1, was found to contain a cluster of genes that are predicted to encode some of these enzymes and was shown to contain two of the enzyme activities and one of the methanogenic coenzymes. Insertion mutants were all unable to grow on C 1 compounds, suggesting that the archaeal enzymes function in aerobic C 1 metabolism. Thus, methylotrophy and methanogenesis involve common genes that cross the bacterial/archaeal boundaries.
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Many archaea-to-bacteria transfers are known for the archaeal methyl synthesis pathway, starting with Methylobacterium extorquens, where genes for the entire methyl synthesis pathway and methanopterin cofactor synthesis were acquired by transfer from archaea.
“Many such archaea‐to‐bacteria transfers are known for the archaeal methyl synthesis pathway, starting with the O2‐dependent bacterial methylotroph Methylobacterium extorquens, where genes for the entire methyl synthesis pathway and methanopterin cofactor synthesis were acquired by transfer from archaea [150].”
From Evidence for corrin biosynthesis in the last universal common ancestor (2025) · cites this paper as [150]
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Martin, W., & Müller, M. (1998) · Nature · doi:10.1038/32096 · Cited by 1,282 · see citing works
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Martin, W., & Russell, M. J. (2003) · Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences · doi:10.1098/rstb.2002.1183 · Cited by 856 · see citing works
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Evidence for corrin biosynthesis in the last universal common ancestor
Modjewski, L. D., Karavaeva, V., Mrnjavac, N., Knopp, M., Martin, W. F., & Sousa, F. L. (2025) · The FEBS Journal · doi:10.1111/febs.17367 · Cited by 15 · see citing works
“…transfers are known for the archaeal methyl synthesis pathway, starting with the O 2 -dependent bacterial methylotroph Methylobacter-ium extorquens , where genes for the entire methyl synthesis pathway and methanopterin cofactor synthesis were acquired by transfer from archaea [150].”
- Authors
- Chistoserdova, L., Vorholt, J. A., Thauer, R. K., & Lidstrom, M. E.
- Affiliations
- University of Washington (US)
- Corresponding author
- Lidstrom, Mary E.
- Venue
- Science
- Published
- 1998-07-03
- Volume
- 281
- Issue
- 5373
- Pages
- 99-102
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2050166373
- Cited by
- 301
- References
- 1
- Citations by year
- 2026: 8 · 2025: 3 · 2024: 6 · 2023: 5 · 2022: 7 · 2021: 7 · 2020: 9 · 2019: 10 · 2018: 10 · 2017: 5 · 2016: 7 · 2015: 11 · 2014: 18 · 2013: 13 · 2012: 12
- FWCI
- 4.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM36296, R01GM036296)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
- Keywords
- methylotrophic bacteria, methanogenic archaea, Methylobacterium extorquens AM1, methylotrophy, methanogenesis, insertion mutants, gene clusters
- MeSH terms
- Methanol, Amino Acid Sequence, Aminohydrolases, Escherichia coli, Biological Evolution, Genes, Bacterial, Molecular Sequence Data, Mutation, NAD, NADP, Oxidation-Reduction, Pterins, Transformation, Bacterial, Sequence Alignment, Gram-Negative Aerobic Rods and Cocci, Euryarchaeota, Genes, Archaeal, Succinic Acid, Hydroxymethyl and Formyl Transferases
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