More Than 200 Genes Required for Methane Formation from H 2 and CO 2 and Energy Conservation Are Present in Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus
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The hydrogenotrophic methanogens Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus can easily be mass cultured. They have therefore been used almost exclusively to study the biochemistry of methanogenesis from H₂ and CO₂, and the genomes of these two model organisms have been sequenced. The close relationship of the two organisms is reflected in their genomic architecture and coding potential. Within the 1,607 protein coding sequences (CDS) in common, we identified approximately 200 CDS required for the synthesis of the enzymes, coenzymes, and prosthetic groups involved in CO₂ reduction to methane and in coupling this process with the phosphorylation of ADP. Approximately 20 additional genes, such as those for the biosynthesis of F(430) and methanofuran and for the posttranslational modifications of the two methyl-coenzyme M reductases, remain to be identified.
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For methanogens, over 200 genes are predicted to be involved in growth on CO2/H2.
“For methanogens for example it has been predicted that over 200 genes are involved in growth on CO2/H2 (Kaster et al., 2011).”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Kaster et al., 2011
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Flagged first, then by citations
Research works printed but not linked: 3
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- [133]Bechard ME Garcia D Greene D Rasche ME Overproduction, characterization, and site-directed mutagenisis of RFAP synthase from the methanogen Methanothermobacter thermoautotrophicus delta H In: Proceedings of the eneral Meeting of the Americam Society for Microbiology 2003
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- Authors
- Kaster, A.-K., Goenrich, M., Seedorf, H., Liesegang, H., Wollherr, A., Gottschalk, G., & Thauer, R. K.
- Affiliations
- Max Planck Society (DE)
- Washington University in St. Louis (US)
- University of Göttingen (DE)
- Corresponding author
- Thauer, Rudolf K.
- Venue
- Archaea
- Publisher
- Wiley
- Published
- 2011
- Volume
- 2011
- Pages
- 1-23
- ISSN
- 1472-3646 (ISSN-L)
- 1472-3654
- PMC
- PMC3087415
- OpenAlex
- W2124148907
- Cited by
- 134
- References
- 170
- Citations by year
- 2026: 10 · 2025: 8 · 2024: 10 · 2023: 8 · 2022: 13 · 2021: 8 · 2020: 7 · 2019: 7 · 2018: 13 · 2017: 5 · 2016: 8 · 2015: 5 · 2014: 12 · 2013: 10 · 2012: 9
- FWCI
- 21.58
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Archaea (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (acceptedVersion, other-oa)
- Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Max Planck Institute for Plasma Physics (submittedVersion)
- GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Niedersächsische Ministerium für Wissenschaft und Kultur
- Max-Planck-Gesellschaft
- Topics
- Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
- Keywords
- Methanothermobacter marburgensis, Methanothermobacter thermautotrophicus, hydrogenotrophic methanogenesis, CO2 methanation, energy conservation, ADP phosphorylation, methyl-coenzyme M reductase, post-translational modification, archaeal genomes, protein-coding sequences
- MeSH terms
- Carbon Dioxide, Energy Metabolism, Hydrogen, Methane, Oxidation-Reduction, Open Reading Frames, Methanobacteriaceae, Genes, Archaeal, Genome, Archaeal, Synteny, Biosynthetic Pathways
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