Cited by 134Anaerobic Digestion and Biogas ProductionMicrobial metabolism and enzyme functionMethane Hydrates and Related Phenomena

More Than 200 Genes Required for Methane Formation from H 2 and CO 2 and Energy Conservation Are Present in Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus

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Kaster, Anne-Kristin, Goenrich, Meike, Seedorf, Henning, Liesegang, Heiko, Wollherr, Antje, Gottschalk, Gerhard, Thauer, Rudolf K.·2011·Archaea

Abstract

The hydrogenotrophic methanogens Methanothermobacter marburgensis and Methanothermobacter thermautotrophicus can easily be mass cultured. They have therefore been used almost exclusively to study the biochemistry of methanogenesis from H₂ and CO₂, and the genomes of these two model organisms have been sequenced. The close relationship of the two organisms is reflected in their genomic architecture and coding potential. Within the 1,607 protein coding sequences (CDS) in common, we identified approximately 200 CDS required for the synthesis of the enzymes, coenzymes, and prosthetic groups involved in CO₂ reduction to methane and in coupling this process with the phosphorylation of ADP. Approximately 20 additional genes, such as those for the biosynthesis of F(430) and methanofuran and for the posttranslational modifications of the two methyl-coenzyme M reductases, remain to be identified.

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  1. [27]Schoenheit P Moll J Thauer RK Growth parameters (KS, μ max, YS) of Methanobacterium thermoautotrophicum Archives of Microbiology 1980 127 1 59 65 120728
  2. [31]Hüster R Thauer RK Pyruvate assimilation by Methanobacterium thermoautotrophicum FEMS Microbiology Letters 1983 19 2-3 207 209
  3. [133]Bechard ME Garcia D Greene D Rasche ME Overproduction, characterization, and site-directed mutagenisis of RFAP synthase from the methanogen Methanothermobacter thermoautotrophicus delta H In: Proceedings of the eneral Meeting of the Americam Society for Microbiology 2003

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Authors
Kaster, A.-K., Goenrich, M., Seedorf, H., Liesegang, H., Wollherr, A., Gottschalk, G., & Thauer, R. K.
Affiliations
  • Max Planck Society (DE)
  • Washington University in St. Louis (US)
  • University of Göttingen (DE)
Corresponding author
Thauer, Rudolf K.
Venue
Archaea
Publisher
Wiley
Published
2011
Volume
2011
Pages
1-23
ISSN
  • 1472-3646 (ISSN-L)
  • 1472-3654
OpenAlex
W2124148907
Cited by
134
References
170
Citations by year
2026: 10 · 2025: 8 · 2024: 10 · 2023: 8 · 2022: 13 · 2021: 8 · 2020: 7 · 2019: 7 · 2018: 13 · 2017: 5 · 2016: 8 · 2015: 5 · 2014: 12 · 2013: 10 · 2012: 9
FWCI
21.58
Type
journal article
Language
en
Open access
Locations
  • Archaea (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (acceptedVersion, other-oa)
  • Digital Commons@Becker (Washington University School of Medicine) (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • GoeScholar The Publication Server of the Georg-August-Universität Göttingen (Georg-August-Universität Göttingen) (submittedVersion, cc-by)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
  • Niedersächsische Ministerium für Wissenschaft und Kultur
  • Max-Planck-Gesellschaft
Topics
  • Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
  • Physical Sciences › Environmental Science › Environmental Chemistry › Methane Hydrates and Related Phenomena
Keywords
Methanothermobacter marburgensis, Methanothermobacter thermautotrophicus, hydrogenotrophic methanogenesis, CO2 methanation, energy conservation, ADP phosphorylation, methyl-coenzyme M reductase, post-translational modification, archaeal genomes, protein-coding sequences
MeSH terms
Carbon Dioxide, Energy Metabolism, Hydrogen, Methane, Oxidation-Reduction, Open Reading Frames, Methanobacteriaceae, Genes, Archaeal, Genome, Archaeal, Synteny, Biosynthetic Pathways

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