The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
A Preorganized Active Site in the Crystal Structure of the Tetrahymena Ribozyme
Abstract
Group I introns possess a single active site that catalyzes the two sequential reactions of self-splicing. An RNA comprising the two domains of the Tetrahymena thermophila group I intron catalytic core retains activity, and the 5.0 angstrom crystal structure of this 247-nucleotide ribozyme is now described. Close packing of the two domains forms a shallow cleft capable of binding the short helix that contains the 5′ splice site. The helix that provides the binding site for the guanosine substrate deviates significantly from A-form geometry, providing a tight binding pocket. The binding pockets for both the 5′ splice site helix and guanosine are formed and oriented in the absence of these substrates. Thus, this large ribozyme is largely preorganized for catalysis, much like a globular protein enzyme.
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A general two-metal-ion mechanism for catalytic RNA
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Structural Features that Give Rise to the Unusual Stability of RNA Hairpins Containing GNRA Loops
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Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure
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Self-citationMetals, Motifs, and Recognition in the Crystal Structure of a 5S rRNA Domain
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Zhuang, X., Bartley, L. E., Babcock, H. P., Russell, R., Ha, T., Herschlag, D., & Chu, S. (2000) · Science · doi:10.1126/science.288.5473.2048 · Cited by 732
Metadata
- Authors
- Golden, B. L., Gooding, A. R., Podell, E. R., & Cech, T. R.
- Venue
- Science
- Published
- 1998-10-09
- Volume
- 282
- Issue
- 5387
- Pages
- 259-264
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2041331047
- Cited by
- 314
- References
- 54
- FWCI
- 11.82
- Type
- journal article
- Language
- en
- Open access
- closed
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- Golden, B. L., Gooding, A. R., Podell, E. R., & Cech, T. R. (1998). A Preorganized Active Site in the Crystal Structure of the Tetrahymena Ribozyme. Science. https://doi.org/10.1126/science.282.5387.259
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
