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Cited by 134Ion Channels and ReceptorsIon channel regulation and functionCalcium signaling and nucleotide metabolism

Single-Channel Recording of a Store-Operated Ca 2+ Channel in Jurkat T Lymphocytes

Kerschbaum, Hubert H., Cahalan, Michael D.·1999·Science
DOI 10.1126/science.283.5403.836
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Abstract

In T lymphocytes, a store-operated calcium ion (Ca 2+ ) entry mechanism termed the calcium release–activated Ca 2+ channel (CRAC channel) underlies the sustained or oscillatory intracellular calcium concentration signal required for interleukin-2 gene expression and cell proliferation. The use of sodium ions as a current carrier enabled single-channel recordings of CRAC channels during activation, inactivation, and blockade of current in the presence of divalent cations. A large conductance of 36 to 40 picosiemens indicates that 100 to 400 CRAC channels are present in T lymphocytes.

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Crossref · 10.1126/science.283.5403.836

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14 of 14 references verified0 citation errors · 0 not checkable

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  • Unlike some SOC channels in higher eukaryotes that are highly permeable to Na+ in the absence of divalent cations, Cch1 is weakly permeable to Na+ and maintains strong Ca2+ selectivity even in high [Na+].

    “These results indicated that unlike some SOC channels in higher eukaryotes that are highly permeable to Na ϩ in the absence of divalent cations, Cch1 is weakly permeable to Na ϩ and maintains a strong Ca 2ϩ selectivity even in the presence of high [Na ϩ ] (4, 27).”

    From Cch1 Restores Intracellular Ca2+ in Fungal Cells during Endoplasmic Reticulum Stress (2010) · cites this paper as [27]

  • Single Cch1 channels exhibited long duration openings with a conductance of about 0.08 picosiemens in 10 mM Ba2+.

    “Single channels exhibited long duration openings with a conductance of ϳ0.08 picosiemens in 10 mM Ba 2ϩ , consistent with the small channel conductance reported for CRAC channels (27).”

    From Cch1 Restores Intracellular Ca2+ in Fungal Cells during Endoplasmic Reticulum Stress (2010) · cites this paper as [27]

  • SOC channels are highly permeable to sodium and other monovalent ions in the absence of divalent cations.

    “One of the distinguishing properties of SOC channels is their highly permeability to sodium ions and other monovalent ions in the absence of divalent cations (27).”

    From Cch1 Restores Intracellular Ca2+ in Fungal Cells during Endoplasmic Reticulum Stress (2010) · cites this paper as [27]

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Authors
Kerschbaum, H. H., & Cahalan, M. D.
Affiliations
  • University of Salzburg (AT)
  • University of California, Irvine (US)
Corresponding author
Cahalan, Michael D.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1999-02-05
Volume
283
Issue
5403
Pages
836-839
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.283.5403.836
OpenAlex
W2054736731
Cited by
134
References
14
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2018: 1 · 2015: 1 · 2014: 1 · 2012: 3
FWCI
11.54
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute of General Medical Sciences (GM-41514, R01GM041514)
  • National Institute of Neurological Disorders and Stroke (NS-14609)
Topics
  • Life Sciences › Neuroscience › Sensory Systems › Ion Channels and Receptors
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
store-operated calcium entry, calcium release-activated calcium channels, CRAC channels, T cells, Jurkat cells, single-channel recording, channel conductance, divalent cations, interleukin-2 gene expression, cell proliferation, intracellular calcium signaling
MeSH terms
Calcium, Calcium Channel Blockers, Egtazic Acid, Electric Conductivity, Gadolinium, Humans, Magnesium, Membrane Potentials, Nickel, Sodium, T-Lymphocytes, Calcium Channels, Inositol 1,4,5-Trisphosphate, Patch-Clamp Techniques, Jurkat Cells

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