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Cited by 936T-cell and B-cell ImmunologyMonoclonal and Polyclonal Antibodies ResearchArtificial Immune Systems Applications

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

A Direct Estimate of the Human αβ T Cell Receptor Diversity

Arstila, T. Petteri, Casrouge, Armanda, Baron, Véronique, Even, Jos, Kanellopoulos, Jean, Kourilsky, Philippe·1999·Science
DOI 10.1126/science.286.5441.958
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Abstract

Generation and maintenance of an effective repertoire of T cell antigen receptors are essential to the immune system, yet the number of distinct T cell receptors (TCRs) expressed by the estimated 10 12 T cells in the human body is not known. In this study, TCR gene amplification and sequencing showed that there are about 10 6 different β chains in the blood, each pairing, on the average, with at least 25 different α chains. In the memory subset, the diversity decreased to 1 × 10 5 to 2 × 10 5 different β chains, each pairing with only a single α chain. Thus, the naı̈ve repertoire is highly diverse, whereas the memory compartment, here one-third of the T cell population, contributes less than 1 percent of the total diversity.

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Research works printed but not linked: 2

  1. Arden B., Clark S. P., Kabelitz D., Mak T. W., Immunogenetics 42, 501 (1995);
  2. Bacterial colonies were lifted on nylon filters (Hybond) lysed denatured fixed and then hybridized overnight at 63°C in 6× concentrated standard saline citrate 1 mM EDTA 0.5% SDS 0.1% bovine serum albumin preboiled salmon sperm DNA (100 μg/ml) with 10 pmol [γ- 32 P]-adenosine-5′-triphosphate–labeled oligonucleotides as recommended by Amersham Pharmacia Biotech and by J. Sambrook E. F. Fritsch T. Maniatis Molecular Cloning (Cold Spring Harbor Laboratory Press Cold Spring Harbor NY ed. 2 1989). The oligonucleotides were 20 to 22 mers each with melting temperature of 64°C. The first oligonucleotide mix consisted of 12 oligonucleotides and in control experiments hybridized to 87% of a mix of bacterial clones known to bear a relevant insert. We also tested two relevant clones separately and in both cases the mix hybridized to >90% of colonies although the strength of the signal was not uniform. Of 28 different clones with unrelated CDR3 sequences none gave a positive signal. The second mix consisted of five oligonucleotides. In random sequencing 19% of the clones in the first sample bore relevant inserts; the second oligonucleotide mix gave a detectable signal in 10% of the clones. We identified one relevant clone not detected by the mix and none of 16 irrelevant clones were positive.

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Metadata

Authors
Arstila, T. P., Casrouge, A., Baron, V., Even, J., Kanellopoulos, J., & Kourilsky, P.
Affiliations
  • Inserm (FR)
  • Unité de Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, 75724 Paris Cedex 15, France.
Corresponding author
Arstila, T. Petteri
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1999-10-29
Volume
286
Issue
5441
Pages
958-961
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.286.5441.958
OpenAlex
W2105381977
Cited by
931
References
30
Citations by year
2026: 25 · 2025: 28 · 2024: 29 · 2023: 24 · 2022: 26 · 2021: 32 · 2020: 57 · 2019: 42 · 2018: 42 · 2017: 38 · 2016: 51 · 2015: 45 · 2014: 42 · 2013: 49 · 2012: 49
FWCI
8.21
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
  • Physical Sciences › Computer Science › Artificial Intelligence › Artificial Immune Systems Applications
Keywords
T cell receptor diversity, alpha beta T cells, T cell receptor repertoire, T cell receptor beta chain, TCR alpha chain, T cell memory, naive T cells, T cell receptor
MeSH terms
Adult, Female, Gene Amplification, Humans, Immunologic Memory, Male, T-Lymphocytes, Genetic Variation, Gene Rearrangement, T-Lymphocyte, Receptors, Antigen, T-Cell, alpha-beta

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