The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Regulation of Protein Secretion Through Controlled Aggregation in the Endoplasmic Reticulum
Abstract
A system for direct pharmacologic control of protein secretion was developed to allow rapid and pulsatile delivery of therapeutic proteins. A protein was engineered so that it accumulated as aggregates in the endoplasmic reticulum. Secretion was then stimulated by a synthetic small-molecule drug that induces protein disaggregation. Rapid and transient secretion of growth hormone and insulin was achieved in vitro and in vivo. A regulated pulse of insulin secretion resulted in a transient correction of serum glucose concentrations in a mouse model of hyperglycemia. This approach may make gene therapy a viable method for delivery of polypeptides that require rapid and regulated delivery.
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Citations
Cites
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Redesigning an FKBP–ligand interface to generate chemical dimerizers with novel specificity
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Self-citationA humanized system for pharmacologic control of gene expression
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Cited by
α-Synuclein impairs macroautophagy: implications for Parkinson’s disease
Winslow, A. R., Chen, C.-W., Corrochano, S., Acevedo-Arozena, A., Gordon, D. E., Peden, A. A., Lichtenberg, M., Menzies, F. M., Ravikumar, B., Imarisio, S., et al. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201003122 · Cited by 825
“The vector (pC4S1-FM4-FCS-hGH) encoding the pharmacologically regulated reporter construct was obtained from ARIAD Pharmaceuticals (Rivera et al., 2000).”
InfluentialQuality control in the endoplasmic reticulum protein factory
Sitia, R., & Braakman, I. (2003) · Nature · doi:10.1038/nature02262 · Cited by 688
Chemically induced proximity in biology and medicine
Stanton, B. Z., Chory, E. J., & Crabtree, G. R. (2018) · Science · doi:10.1126/science.aao5902 · Cited by 475
FKBP Ligands—Where We Are and Where to Go?
Kolos, J. M., Voll, A. M., Bauder, M., & Hausch, F. (2018) · Frontiers in Pharmacology · doi:10.3389/fphar.2018.01425 · Cited by 158
Plasma membrane protein polarity and trafficking in RPE cells: past, present and future
Lehmann, G. L., Benedicto, I., Philp, N. J., & Rodriguez-Boulan, E. (2014) · Experimental Eye Research · doi:10.1016/j.exer.2014.04.021 · Cited by 122
Metadata
- Authors
- Rivera, V. M., Wang, X., Wardwell, S., Courage, N. L., Volchuk, A., Keenan, T., Holt, D. A., Gilman, M., Orci, L., Cerasoli, F., Rothman, J. E., & Clackson, T.
- Venue
- Science
- Published
- 2000-02-04
- Volume
- 287
- Issue
- 5454
- Pages
- 826-830
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2113267766
- Cited by
- 339
- References
- 33
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- closed
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- Rivera, V. M., Wang, X., Wardwell, S., Courage, N. L., Volchuk, A., Keenan, T., Holt, D. A., Gilman, M., Orci, L., Cerasoli, F., Rothman, J. E., & Clackson, T. (2000). Regulation of Protein Secretion Through Controlled Aggregation in the Endoplasmic Reticulum. Science. https://doi.org/10.1126/science.287.5454.826
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
