The DOI matches its Crossref record; 41 of the 41 references it cites were found in a registry or an index.
General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme
Abstract
Many protein enzymes use general acid-base catalysis as a way to increase reaction rates. The amino acid histidine is optimized for this function because it has a p K a (where K a is the acid dissociation constant) near physiological pH. The RNA enzyme (ribozyme) from hepatitis delta virus catalyzes self-cleavage of a phosphodiester bond. Reactivity-pH profiles in monovalent or divalent cations, as well as distance to the leaving-group oxygen, implicate cytosine 75 (C75) of the ribozyme as the general acid and ribozyme-bound hydrated metal hydroxide as the general base in the self-cleavage reaction. Moreover, C75 has a p K a perturbed to neutrality, making it “histidine-like.” Anticooperative interaction is observed between protonated C75 and a metal ion, which serves to modulate the p K a of C75. General acid-base catalysis expands the catalytic repertoire of RNA and may provide improved rate acceleration.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Principles of Nucleic Acid Structure
(2008) · Elsevier eBooks · doi:10.1016/b978-0-12-369507-9.x5001-8 · Cited by 4,504
Principles of Nucleic Acid Structure
Saenger, W. (1984) · Springer Advanced Texts in Chemistry · doi:10.1007/978-1-4612-5190-3 · Cited by 4,150
Hydrogen Ion Buffers for Biological Research*
Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S., & Singh, R. M. M. (1966) · Biochemistry · doi:10.1021/bi00866a011 · Cited by 2,762
Control of memory formation through regulated expression of a CaMKII transgene
Mayford, M., Bach, M. E., Huang, Y.-Y., Wang, L., Hawkins, R. D., & Kandel, E. R. (1996) · Science · doi:10.1126/science.274.5293.1678 · Cited by 1,550
Crystal Structure of a Group I Ribozyme Domain: Principles of RNA Packing
Cate, J. H., Gooding, A. R., Podell, E., Zhou, K., Golden, B. L., Kundrot, C. E., Cech, T. R., & Doudna, J. A. (1996) · Science · doi:10.1126/science.273.5282.1678 · Cited by 1,248
Ferrin, T. E., Huang, C. C., Jarvis, L. E., & Langridge, R. (1988) · Journal of Molecular Graphics · doi:10.1016/0263-7855(88)80054-7 · Cited by 950
Binding Energy, Specificity, and Enzymic Catalysis: The Circe Effect
Jencks, W. P. (1975) · Advances in Enzymology - and Related Areas of Molecular Biology · doi:10.1002/9780470122884.ch4 · Cited by 854
Crystal structure of a hepatitis delta virus ribozyme
Ferré-D'Amaré, A. R., Zhou, K., & Doudna, J. A. (1998) · Nature · doi:10.1038/26912 · Cited by 785
Oivanen, M., Kuusela, S., & Lönnberg, H. (1998) · Chemical Reviews · doi:10.1021/cr960425x · Cited by 438
[29] Solvent isotope effects on enzyme systems
Barbara Schowen, K., & Schowen, R. L. (1982) · Methods in Enzymology · doi:10.1016/s0076-6879(82)87031-6 · Cited by 404
Cited by
The Structural Basis of Ribosome Activity in Peptide Bond Synthesis
Nissen, P., Hansen, J., Ban, N., Moore, P. B., & Steitz, T. A. (2000) · Science · doi:10.1126/science.289.5481.920 · Cited by 2,269
Asymmetric Catalysis by Chiral Hydrogen‐Bond Donors
Taylor, M. S., & Jacobsen, E. N. (2006) · Angewandte Chemie International Edition · doi:10.1002/anie.200503132 · Cited by 1,906
The chemical repertoire of natural ribozymes
Doudna, J. A., & Cech, T. R. (2002) · Nature · doi:10.1038/418222a · Cited by 745
RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview
Šponer, J., Bussi, G., Krepl, M., Banáš, P., Bottaro, S., Cunha, R. A., Gil-Ley, A., Pinamonti, G., Poblete, S., Jurečka, P., et al. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00427 · Cited by 599
Crystal structure of a hairpin ribozyme–inhibitor complex with implications for catalysis
Rupert, P. B., & Ferré-D'Amaré, A. R. (2001) · Nature · doi:10.1038/35071009 · Cited by 447
Metadata
- Authors
- Nakano, S.-I., Chadalavada, D. M., & Bevilacqua, P. C.
- Venue
- Science
- Published
- 2000-02-25
- Volume
- 287
- Issue
- 5457
- Pages
- 1493-1497
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1974000520
- Cited by
- 385
- References
- 41
- FWCI
- 9.80
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme. https://app.cochat.ai/papers/10.1126/science.287.5457.1493
- The paper
- Nakano, S.-I., Chadalavada, D. M., & Bevilacqua, P. C. (2000). General Acid-Base Catalysis in the Mechanism of a Hepatitis Delta Virus Ribozyme. Science. https://doi.org/10.1126/science.287.5457.1493
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
