The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The Use of a Charge-Coupled Device for Quantitative Optical Microscopy of Biological Structures
Abstract
The properties of a charge-coupled device (CCD) and its application to the high-resolution analysis of biological structures by optical microscopy are described. The CCD, with its high resolution, high sensitivity, wide dynamic range, photometric accuracy, and geometric stability, can provide data of such high quality that quantitative analysis on two- and three-dimensional microscopic images is possible. For example, the three-dimensional imaging properties of an epifluorescence microscope have been quantitatively determined with the CCD. This description of the imaging properties of the microscope, and the high-quality image data provided by the CCD, allow sophisticated computational image processing methods to be used that greatly improve the effective resolution obtainable for biological structures. Image processing techniques revealed fine substructures in Drosophila embryonic diploid chromosomes in two and three dimensions. The same approach can be extended to structures as small as yeast chromosomes or to other problems in structural cell biology.
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Cites
The embryonic cell lineage of the nematode Caenorhabditis elegans
Sulston, J. E., Schierenberg, E., White, J. G., & Thomson, J. N. (1983) · Developmental Biology · doi:10.1016/0012-1606(83)90201-4 · Cited by 4,199
Cited by
Chapter 13 Fluorescence Microscopy in Three Dimensions
Agard, D. A., Hiraoka, Y., Shaw, P., & Sedat, J. W. (1989) · Methods in Cell Biology · doi:10.1016/s0091-679x(08)60986-3 · Cited by 649
Self-citationTheory of Midinfrared Absorption Microspectroscopy: I. Homogeneous Samples
Davis, B. J., Carney, P. S., & Bhargava, R. (2010) · Analytical Chemistry · doi:10.1021/ac902067p · Cited by 115
Metadata
- Authors
- Hiraoka, Y., Sedat, J. W., & Agard, D. A.
- Venue
- Science
- Published
- 1987-10-02
- Volume
- 238
- Issue
- 4823
- Pages
- 36-41
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2025224752
- Cited by
- 300
- References
- 1
- FWCI
- 32.21
- Type
- journal article
- Language
- en
- Open access
- closed
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- Hiraoka, Y., Sedat, J. W., & Agard, D. A. (1987). The Use of a Charge-Coupled Device for Quantitative Optical Microscopy of Biological Structures. Science. https://doi.org/10.1126/science.3116667
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