The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.
Germline Transformation Used to Define Key Features of Heat-Shock Response Elements
Abstract
The heat-shock consensus element (HSE), CTNGAANNTTCNAG, is found in multiple copies upstream of all heat-shock genes. Here, the sequence requirements for heat-shock induction are tested by Drosophila germline transformation with an hsp 70- lacZ gene fused to a pair of synthetic HSEs. Certain single-base substitutions in either HSE cause a dramatic reduction (fortyfold) in expression. Surprisingly, variations in sequences immediately flanking the HSEs also reduced levels of induction. One such variant that contains two perfect 14-base pair HSEs, which are correctly spaced relative to each other and the TATA box, retained only 7% of wild type-induced expression. These and additional analyses indicate that the heat-shock regulatory element includes sequences beyond the 14-base pair HSE and may be better described as a dimer of a 10-base pair sequence, NTTCNNGAAN.
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Heat shock factors: integrators of cell stress, development and lifespan
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Regulation of H SF 1 Function in the Heat Stress Response: Implications in Aging and Disease
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Small heat shock proteins and stress tolerance in plants
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Metadata
- Authors
- Xiao, H., & Lis, J. T.
- Venue
- Science
- Published
- 1988-03-04
- Volume
- 239
- Issue
- 4844
- Pages
- 1139-1142
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2012015460
- Cited by
- 437
- References
- 15
- FWCI
- 13.85
- Type
- journal article
- Language
- en
- Open access
- closed
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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