Feedback control of mitosis in budding yeast
Abstract
We have investigated the feedback control that prevents cells with incompletely assembled spindles from leaving mitosis. We isolated budding yeast mutants sensitive to the anti-microtubule drug benomyl. Mitotic arrest-deficient (mad) mutants are the subclass of benomyl-sensitive mutants in which the completion of mitosis is not delayed in the presence of benomyl and that die as a consequence of their premature exit from mitosis. A number of properties of the mad mutants indicate that they are defective in the feedback control over the exit from mitosis: their killing by benomyl requires passage through mitosis; their benomyl sensitivity can be suppressed by an independent method for delaying the exit from mitosis; they have normal microtubules; and they have increased frequencies of chromosome loss. We cloned MAD2, which encodes a putative calcium-binding protein whose disruption is lethal. We discuss the role of feedback controls in coordinating events in the cell cycle.
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A cell cycle delay may result when a mutation activates a checkpoint pathway which slows cell cycle progression, allowing the cell to correct an otherwise rate-limiting defect to mask the phenotypic effect normally associated with a second mutation.
“A cell cycle delay may result when a mutation activates a checkpoint pathway which slows cell cycle progression, allowing the cell to correct an otherwise rate-limiting defect to mask the phenotypic effect normally associated with a second mutation (36).”
From A global genetic interaction network maps a wiring diagram of cellular function (2016) · cites this paper as [36]
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- Authors
- Li, R., & Murray, A. W.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1991-08
- Volume
- 66
- Issue
- 3
- Pages
- 519-531
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1973916918
- Cited by
- 1,174
- References
- 1
- Citations by year
- 2026: 10 · 2025: 11 · 2024: 18 · 2023: 13 · 2022: 18 · 2021: 16 · 2020: 22 · 2019: 15 · 2018: 24 · 2017: 22 · 2016: 27 · 2015: 24 · 2014: 44 · 2013: 31 · 2012: 33
- FWCI
- 16.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- mitosis, feedback control, exit from mitosis, spindle assembly, benomyl sensitivity, Mad2, calcium-binding proteins, chromosome loss, cell cycle regulation, Saccharomyces cerevisiae
- MeSH terms
- Amino Acid Sequence, Base Sequence, Benomyl, Calcium-Binding Proteins, Carrier Proteins, Cell Cycle, Cell Nucleus, Cloning, Molecular, Cytoskeleton, DNA Mutational Analysis, DNA, Fungal, Feedback, Fungal Proteins, Genes, Fungal, Protamine Kinase, Microtubules, Mitosis, Spindle Apparatus, Molecular Sequence Data, Mutation, Nuclear Proteins, Saccharomyces cerevisiae, Chromosomes, Fungal, Cell Cycle Proteins, Saccharomyces cerevisiae Proteins, Mad2 Proteins
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