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URF6, Last Unidentified Reading Frame of Human mtDNA, Codes for an NADH Dehydrogenase Subunit

Chomyn, Anne, Cleeter, Michael W. J., Ragan, C. Jan, Riley, Marcia, Doolittle, Russell F., Attardi, Giuseppe·1986·Science
DOI 10.1126/science.3764430
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Abstract

The polypeptide encoded in URF6, the last unassigned reading frame of human mitochondrial DNA, has been identified with antibodies to peptides predicted from the DNA sequence. Antibodies prepared against highly purified respiratory chain NADH dehydrogenase from beef heart or against the cytoplasmically synthesized 49-kilodalton iron-sulfur subunit isolated from this enzyme complex, when added to a deoxycholate or a Triton X-100 mitochondrial lysate of HeLa cells, specifically precipitated the URF6 product together with the six other URF products previously identified as subunits of NADH dehydrogenase. These results strongly point to the URF6 product as being another subunit of this enzyme complex. Thus, almost 60% of the protein coding capacity of mammalian mitochondrial DNA is utilized for the assembly of the first enzyme complex of the respiratory chain. The absence of such information in yeast mitochondrial DNA dramatizes the variability in gene content of different mitochondrial genomes.

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Research works printed but not linked: 47

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Metadata

Authors
Chomyn, A., Cleeter, M. W. J., Ragan, C. J., Riley, M., Doolittle, R. F., & Attardi, G.
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
1986-10-31
Volume
234
Issue
4776
Pages
614-618
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.3764430
OpenAlex
W2137999969
Cited by
349
References
0
FWCI
9.12
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: URF6, Last Unidentified Reading Frame of Human mtDNA, Codes for an NADH Dehydrogenase Subunit. https://app.cochat.ai/papers/10.1126/science.3764430
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Chomyn, A., Cleeter, M. W. J., Ragan, C. J., Riley, M., Doolittle, R. F., & Attardi, G. (1986). URF6, Last Unidentified Reading Frame of Human mtDNA, Codes for an NADH Dehydrogenase Subunit. Science. https://doi.org/10.1126/science.3764430

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