The DOI matches its Crossref record.
Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation
Abstract
Endothelial cells from human blood vessels were cultured in vitro, with doubling times of 17 to 21 hours for 42 to 79 population doublings. Cloned human endothelial cell strains were established for the first time and had similar proliferative capacities. This vigorous cell growth was achieved by addition of heparin to culture medium containing reduced concentrations of endothelial cell growth factor. The routine cloning and long-term culture of human endothelial cells will facilitate studying the human endothelium in vitro.
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Citations
Cited by
Folkman, J., & Klagsbrun, M. (1987) · Science · doi:10.1126/science.2432664 · Cited by 3,491
Yayon, A., Klagsbrun, M., Esko, J. D., Leder, P., & Ornitz, D. M. (1991) · Cell · doi:10.1016/0092-8674(91)90512-w · Cited by 2,440
Schmidt, A. M., Hori, O., Chen, J. X., Li, J. F., Crandall, J., Zhang, J., Cao, R., Yan, S. D., Brett, J., & Stern, D. (1995) · Journal of Clinical Investigation · doi:10.1172/jci118175 · Cited by 896
DeCaprio, J. A., Ludlow, J. W., Lynch, D., Furukawa, Y., Griffin, J., Piwnica-Worms, H., Huang, C.-M., & Livingston, D. M. (1989) · Cell · doi:10.1016/0092-8674(89)90507-2 · Cited by 897
Heparin protects basic and acidic FGF from inactivation
Gospodarowicz, D., & Cheng, J. (1986) · Journal of Cellular Physiology · doi:10.1002/jcp.1041280317 · Cited by 853
Metadata
- Authors
- Thornton, S. C., Mueller, S. N., & Levine, E. M.
- Venue
- Science
- Published
- 1983-11-11
- Volume
- 222
- Issue
- 4624
- Pages
- 623-625
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W1964537647
- Cited by
- 793
- References
- 0
- FWCI
- 15.49
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation. https://app.cochat.ai/papers/10.1126/science.6635659
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- Thornton, S. C., Mueller, S. N., & Levine, E. M. (1983). Human Endothelial Cells: Use of Heparin in Cloning and Long-Term Serial Cultivation. Science. https://doi.org/10.1126/science.6635659
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
