The DOI matches its Crossref record.
Generation and Assembly of Secretory Antibodies in Plants
Abstract
Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, a hybrid immunoglobulin A-G heavy chain, a murine joining chain, and a rabbit secretory component, respectively. Successive sexual crosses between these plants and filial recombinants resulted in plants that expressed all four protein chains simultaneously. These chains were assembled into a functional, high molecular weight secretory immunoglobulin that recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble secretory antibodies, whereas two different cell types are required in mammals. Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy. Plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules.
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Citations
Cited by
INTERACTION OF ANTIGENS AND ANTIBODIES AT MUCOSAL SURFACES
Lamm, M. E. (1997) · Annual Review of Microbiology · doi:10.1146/annurev.micro.51.1.311 · Cited by 429
Expression and assembly of a fully active antibody in algae
Mayfield, S. P., Franklin, S. E., & Lerner, R. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237108100 · Cited by 344
Genetically modified plants and human health
Key, S., Ma, J. K.-C., & Drake, P. M. (2008) · Journal of the Royal Society of Medicine · doi:10.1258/jrsm.2008.070372 · Cited by 197
Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast
Manuell, A. L., Beligni, M. V., Elder, J. H., Siefker, D. T., Tran, M., Weber, A., McDonald, T. L., & Mayfield, S. P. (2007) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2007.00249.x · Cited by 154
Delivery of Multiple Transgenes to Plant Cells
Dafny-Yelin, M., & Tzfira, T. (2007) · Plant Physiology · doi:10.1104/pp.107.106104 · Cited by 136
Metadata
- Authors
- Ma, J. K.-C., Hiatt, A., Hein, M., Vine, N. D., Wang, F., Stabila, P., van Dolleweerd, C., Mostov, K., & Lehner, T.
- Venue
- Science
- Published
- 1995-05-05
- Volume
- 268
- Issue
- 5211
- Pages
- 716-719
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2029625075
- Cited by
- 612
- References
- 0
- FWCI
- 32.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Ma, J. K.-C., Hiatt, A., Hein, M., Vine, N. D., Wang, F., Stabila, P., van Dolleweerd, C., Mostov, K., & Lehner, T. (1995). Generation and Assembly of Secretory Antibodies in Plants. Science. https://doi.org/10.1126/science.7732380
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
