The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Expression and assembly of a fully active antibody in algae
Open accessAbstract
Although combinatorial antibody libraries have solved the problem of access to large immunological repertoires, efficient production of these complex molecules remains a problem. Here we demonstrate the efficient expression of a unique large single-chain (lsc) antibody in the chloroplast of the unicellular, green alga, Chlamydomonas reinhardtii . We achieved high levels of protein accumulation by synthesizing the lsc gene in chloroplast codon bias and by driving expression of the chimeric gene using either of two C. reinhardtii chloroplast promoters and 5′ and 3′ RNA elements. This lsc antibody, directed against glycoprotein D of the herpes simplex virus, is produced in a soluble form by the alga and assembles into higher order complexes in vivo . Aside from dimerization by disulfide bond formation, the antibody undergoes no detectable posttranslational modification. We further demonstrate that accumulation of the antibody can be modulated by the specific growth regime used to culture the alga, and by the choice of 5′ and 3′ elements used to drive expression of the antibody gene. These results demonstrate the utility of alga as an expression platform for recombinant proteins, and describe a new type of single chain antibody containing the entire heavy chain protein, including the Fc domain.
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Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
Molecular cloning: A laboratory manual
Deininger, P. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,682
Gorman, D. S., & Levine, R. P. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.6.1665 · Cited by 1,681
(1989) · Elsevier eBooks · doi:10.1016/c2009-0-02778-0 · Cited by 1,128
Production of antibodies in transgenic plants
Hiatt, A., Caffferkey, R., & Bowdish, K. (1989) · Nature · doi:10.1038/342076a0 · Cited by 1,059
Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles
Boynton, J. E., Gillham, N. W., Harris, E. H., Hosler, J. P., Johnson, A. M., Jones, A. R., Randolph-Anderson, B. L., Robertson, D., Klein, T. M., Shark, K. B., et al. (1988) · Science · doi:10.1126/science.2897716 · Cited by 962
Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides
Stemmer, W. P. C., Crameri, A., Ha, K. D., Brennan, T. M., & Heyneker, H. L. (1995) · Gene · doi:10.1016/0378-1119(95)00511-4 · Cited by 728
Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico
Quist, D., & Chapela, I. H. (2001) · Nature · doi:10.1038/35107068 · Cited by 628
Generation and Assembly of Secretory Antibodies in Plants
Ma, J. K.-C., Hiatt, A., Hein, M., Vine, N. D., Wang, F., Stabila, P., van Dolleweerd, C., Mostov, K., & Lehner, T. (1995) · Science · doi:10.1126/science.7732380 · Cited by 612
High-yield production of a human therapeutic protein in tobacco chloroplasts
Staub, J. M., Garcia, B., Graves, J., Hajdukiewicz, P. T. J., Hunter, P., Nehra, N., Paradkar, V., Schlittler, M., Carroll, J. A., Spatola, L., et al. (2000) · Nature Biotechnology · doi:10.1038/73796 · Cited by 486
Cited by
Walker, T. L., Purton, S., Becker, D. K., & Collet, C. (2005) · Plant Cell Reports · doi:10.1007/s00299-005-0004-6 · Cited by 299
Advances in the Production of High-Value Products by Microalgae
Leu, S., & Boussiba, S. (2014) · Industrial Biotechnology · doi:10.1089/ind.2013.0039 · Cited by 253
M. U., N., Mehar, J. G., Mudliar, S. N., & Shekh, A. Y. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12500 · Cited by 236
Chlamydomonas reinhardtii chloroplasts as protein factories
Mayfield, S. P., Manuell, A. L., Chen, S., Wu, J., Tran, M., Siefker, D., Muto, M., & Marin-Navarro, J. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.02.001 · Cited by 201
Self-citationGenetically modified plants and human health
Key, S., Ma, J. K.-C., & Drake, P. M. (2008) · Journal of the Royal Society of Medicine · doi:10.1258/jrsm.2008.070372 · Cited by 197
Metadata
- Authors
- Mayfield, S. P., Franklin, S. E., & Lerner, R. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2003-01-08
- Volume
- 100
- Issue
- 2
- Pages
- 438-442
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2039589107
- Cited by
- 344
- References
- 23
- FWCI
- 11.92
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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