Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Molecular cloning: A laboratory manual

Deininger, P. L. (1990) · Analytical Biochemistry · doi:10.1016/0003-2697(90)90595-z · Cited by 84,684 · see citing works

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

Studier, F. W., & Moffatt, B. A. (1986) · Journal of Molecular Biology · doi:10.1016/0022-2836(86)90385-2 · Cited by 6,372 · see citing works

Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.

Gorman, D. S., & Levine, R. P. (1965) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.54.6.1665 · Cited by 1,685 · see citing works

Recombinant protein folding and misfolding in Escherichia coli

Baneyx, F., & Mujacic, M. (2004) · Nature Biotechnology · doi:10.1038/nbt1029 · Cited by 1,488 · see citing works

The Chlamydomonas sourcebook: a comprehensive guide to biology and laboratory use

(1990) · Choice Reviews Online · doi:10.5860/choice.27-2713 · Cited by 1,075 · see citing works

Production of antibodies in transgenic plants

Hiatt, A., Caffferkey, R., & Bowdish, K. S. (1989) · Nature · doi:10.1038/342076a0 · Cited by 1,065 · see citing works

Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides

Stemmer, W. P. C., Crameri, A., Ha, K. D., Brennan, T. M., & Heyneker, H. L. (1995) · Gene · doi:10.1016/0378-1119(95)00511-4 · Cited by 732 · see citing works

C HLAMYDOMONAS AS A M ODEL O RGANISM

Harris, E. H. (2001) · Annual Review of Plant Physiology and Plant Molecular Biology · doi:10.1146/annurev.arplant.52.1.363 · Cited by 711 · see citing works

Plant-based production of biopharmaceuticals

Fischer, R., Stöger, E., Schillberg, S., Christou, P., & Twyman, R. M. (2004) · Current Opinion in Plant Biology · doi:10.1016/j.pbi.2004.01.007 · Cited by 634 · see citing works

Transgenic DNA introgressed into traditional maize landraces in Oaxaca, Mexico

Quist, D., & Chapela, I. H. (2001) · Nature · doi:10.1038/35107068 · Cited by 629 · see citing works

Generation and Assembly of Secretory Antibodies in Plants

K‐C., J., Hiatt, A., Hein, M. B., Vine, N. D., Wang, F., Stabila, P. F., van Dolleweerd, C. J., Mostov, K. E., & Lehner, T. (1995) · Science · doi:10.1126/science.7732380 · Cited by 612 · see citing works

High-yield production of a human therapeutic protein in tobacco chloroplasts

Staub, J. M., Garcia, B. H., Graves, J., Hajdukiewicz, P. T. J., Hunter, P., Nehra, N. S., Paradkar, V. M., Schlittler, M. R., Carroll, J. A., Spatola, L. A., et al. (2000) · Nature Biotechnology · doi:10.1038/73796 · Cited by 486 · see citing works

Expression and assembly of a fully active antibody in algae

Mayfield, S. P., Franklin, S. E., & Lerner, R. A. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0237108100 · Cited by 345 · see citing works

Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum

McDonald, T. L., Larson, M. A., Mack, D. R., & Weber, A. M. (2001) · Veterinary Immunology and Immunopathology · doi:10.1016/s0165-2427(01)00380-4 · Cited by 218 · see citing works

Preparative expression of secreted proteins in bacteria: status report and future prospects

Georgiou, G., & Segatori, L. (2005) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2005.07.008 · Cited by 207 · see citing works

When Transgenes Wander, Should We Worry?

Ellstrand, N. C. (2001) · Plant Physiology · doi:10.1104/pp.125.4.1543 · Cited by 205 · see citing works

Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast

Franklin, S. E., Ngo, B. X., Efuet, E. T., & Mayfield, S. P. (2002) · The Plant Journal · doi:10.1046/j.1365-313x.2002.01319.x · Cited by 194 · see citing works

Production of biopharmaceuticals and vaccines in plants via the chloroplast genome

Daniell, H. (2006) · Biotechnology Journal · doi:10.1002/biot.200600145 · Cited by 178 · see citing works

Expression of Bacillus anthracis protective antigen in transgenic chloroplasts of tobacco, a non-food/feed crop

Watson, J., Koya, V., Leppla, S. H., & Daniell, H. (2004) · Vaccine · doi:10.1016/j.vaccine.2004.01.069 · Cited by 173 · see citing works

Chloroplast Biogenesis of Photosystem II Cores Involves a Series of Assembly-Controlled Steps That Regulate Translation

Minai, L., Wostrikoff, K., Wollman, F.-A., & Choquet, Y. (2005) · The Plant Cell · doi:10.1105/tpc.105.037705 · Cited by 141 · see citing works

Biogenesis of PSI involves a cascade of translational autoregulation in the chloroplast of Chlamydomonas

Wostrikoff, K., Girard‐Bascou, J., Wollman, F.-A., & Choquet, Y. (2004) · The EMBO Journal · doi:10.1038/sj.emboj.7600266 · Cited by 115 · see citing works

Proteomic Characterization of the Chlamydomonas reinhardtii Chloroplast Ribosome

Yamaguchi, K., Beligni, M. V., Prieto, S., Haynes, P. A., McDonald, W. H., Yates, J. R., & Mayfield, S. P. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m301934200 · Cited by 114 · see citing works

Plant biopharming of monoclonal antibodies

Ko, K., & Koprowski, H. (2005) · Virus Research · doi:10.1016/j.virusres.2005.03.016 · Cited by 112 · see citing works

Plant cell factories and mucosal vaccines

Walmsley, A. M., & Arntzen, C. J. (2003) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(03)00026-0 · Cited by 111 · see citing works

Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes

Barnes, D., Franklin, S. E., Schultz, J., Henry, R. E., Brown, E. C., Coragliotti, A., & Mayfield, S. P. (2005) · Molecular Genetics and Genomics · doi:10.1007/s00438-005-0055-y · Cited by 105 · see citing works

Codon usage tabulated from the international DNA sequence databases; its status 1999

Nakamura, Y., Gojobori, T., & Ikemura, T. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.1.292 · Cited by 101 · see citing works

Uncorking the biomanufacturing bottleneck

Dove, A. (2002) · Nature Biotechnology · doi:10.1038/nbt0802-777 · Cited by 96 · see citing works

Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast

Mayfield, S. P., & Schultz, J. (2004) · The Plant Journal · doi:10.1046/j.1365-313x.2003.01965.x · Cited by 95 · see citing works

Recent developments in the production of human therapeutic proteins in eukaryotic algae

Franklin, S. E., & Mayfield, S. P. (2005) · Expert Opinion on Biological Therapy · doi:10.1517/14712598.5.2.225 · Cited by 92 · see citing works

Antibodies and gene therapy: teaching old ‘magic bullets’ new tricks

Sanz, L., Blanco, B., & Álvarez‐Vallina, L. (2003) · Trends in Immunology · doi:10.1016/j.it.2003.12.001 · Cited by 90 · see citing works

Antibody processing and engineering in plants, and new strategies for vaccine production

K‐C., J., Drake, P. M. W., Chargelegue, D., Obregón, P., & Prada, A. (2004) · Vaccine · doi:10.1016/j.vaccine.2004.11.011 · Cited by 84 · see citing works

Human serum amyloid A3 peptide enhances intestinal MUC3 expression and inhibits EPEC adherence

Larson, M. A., Wei, S. H., Weber, A. M., Mack, D. R., & McDonald, T. L. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02901-7 · Cited by 73 · see citing works

Experiences of virus, retrovirus and retrovirus-like particles in Chinese hamster ovary (CHO) and hybridoma cells used for production of protein therapeutics.

Adamson, S. R. (1998) · PubMed · Cited by 30 · see citing works

Protein expression in plastids

Heifetz, P. B. (2001) · Current Opinion in Plant Biology · doi:10.1016/s1369-5266(00)00153-9 · Cited by 24 · see citing works

[13] Translational regulation of chloroplast gene expression in chlamydomonas reinhardtii

Cohen, A., Yohn, C. B., Bruick, R. K., & Mayfield, S. P. (1998) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(98)97015-x · Cited by 19 · see citing works

Biofuels from algae: Challenges and potential

Hannon, M., Gimpel, J., Tran, M., Rasala, B., & Mayfield, S. (2010) · Biofuels · doi:10.4155/bfs.10.44 · Cited by 813 · see citing works

Renewable fuels from algae: An answer to debatable land based fuels

Singh, A., Nigam, P. S. N., & Murphy, J. D. (2010) · Bioresource Technology · doi:10.1016/j.biortech.2010.06.032 · Cited by 661 · see citing works

A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution

Rosenberg, J. N., Oyler, G. A., Wilkinson, L., & Betenbaugh, M. J. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.07.008 · Cited by 631 · see citing works

Microalgae as a future food source

Torres-Tiji, Y., Fields, F. J., & Mayfield, S. P. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107536 · Cited by 559 · see citing works

Engineering Plastid Genomes: Methods, Tools, and Applications in Basic Research and Biotechnology

Bock, R. (2014) · Annual Review of Plant Biology · doi:10.1146/annurev-arplant-050213-040212 · Cited by 355 · see citing works

Micro-algae come of age as a platform for recombinant protein production

Specht, E. A., Miyake-Stoner, S. J., & Mayfield, S. P. (2010) · Biotechnology Letters · doi:10.1007/s10529-010-0326-5 · Cited by 304 · see citing works

Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses

Rasala, B. A., & Mayfield, S. P. (2014) · Photosynthesis Research · doi:10.1007/s11120-014-9994-7 · Cited by 289 · see citing works

Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production

Jagadevan, S., Banerjee, A., Banerjee, C., Guria, C., Tiwari, R., Baweja, M., & Shukla, P. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1181-1 · Cited by 275 · see citing works

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

Rasala, B. A., Muto, M., Lee, P. A., Jager, M., Cardoso, R. M. F., Behnke, C. A., Kirk, P. B., Hokanson, C. A., Crea, R., Mendez, M. J., et al. (2010) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2010.00503.x · Cited by 271 · see citing works

<i>Chlamydomonas</i> as a model for biofuels and bio‐products production

Scranton, M. A., Ostrand, J. T., Fields, F. J., & Mayfield, S. P. (2015) · The Plant Journal · doi:10.1111/tpj.12780 · Cited by 267 · see citing works

The Potential for Microalgae as Bioreactors to Produce Pharmaceuticals

Yan, N. J., Fan, C., Chen, Y., & Hu, Z. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms17060962 · Cited by 255 · see citing works

Recent Advances in Microalgal Bioactives for Food, Feed, and Healthcare Products: Commercial Potential, Market Space, and Sustainability

M. U., N., Mehar, J. G., Mudliar, S. N., & Shekh, A. Y. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12500 · Cited by 236 · see citing works

Transgene Expression in Microalgae—From Tools to Applications

Doron, L., Segal, N., & Shapira, M. (2016) · Frontiers in Plant Science · doi:10.3389/fpls.2016.00505 · Cited by 235 · see citing works

Establishing Chlamydomonas reinhardtii as an industrial biotechnology host

Scaife, M. A., Nguyen, G. T. D. T., Rico, J. J. R., Lambert, D., Helliwell, K. E., & Smith, A. G. (2015) · The Plant Journal · doi:10.1111/tpj.12781 · Cited by 228 · see citing works

Valorization of greenhouse carbon dioxide emissions into value-added products by catalytic processes

Olajire, A. A. (2013) · Journal of CO2 Utilization · doi:10.1016/j.jcou.2013.10.004 · Cited by 213 · see citing works

Plastid Biotechnology: Food, Fuel, and Medicine for the 21st Century

Maliga, P., & Bock, R. (2011) · PLANT PHYSIOLOGY · doi:10.1104/pp.110.170969 · Cited by 205 · see citing works

Chlamydomonas reinhardtii chloroplasts as protein factories

Mayfield, S. P., Manuell, A. L., Chen, S., Wu, J., Tran, M., Siefker, D., Muto, M., & Marin-Navarro, J. (2007) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2007.02.001 · Cited by 201 · see citing works

Green factory: Plants as bioproduction platforms for recombinant proteins

Xu, J., Dolan, M. C., Medrano, G., Cramer, C. L., & Weathers, P. J. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.08.020 · Cited by 191 · see citing works

Strategies for high-level recombinant protein expression in transgenic microalgae: A review

Potvin, G., & Zhang, Z. (2010) · Biotechnology Advances · doi:10.1016/j.biotechadv.2010.08.006 · Cited by 188 · see citing works

Production of unique immunotoxin cancer therapeutics in algal chloroplasts

Tran, M., Van, C., Barrera, D. J., Pettersson, P. L., Peinado, C. D., Bui, J. D., & Mayfield, S. P. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1214638110 · Cited by 186 · see citing works

Factors effecting expression of vaccines in microalgae

Surzycki, R., Greenham, K., Kitayama, K., Dibal, F., Wagner, R., Rochaix, J.-D., Ajam, T., & Surzycki, S. (2009) · Biologicals · doi:10.1016/j.biologicals.2009.02.005 · Cited by 185 · see citing works

Hypes, hopes, and the way forward for microalgal biotechnology

Barbosa, M. J., Janssen, M. G. J., Südfeld, C., D’Adamo, S., & Wijffels, R. H. (2023) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.12.017 · Cited by 184 · see citing works

Synthesis and assembly of a full‐length human monoclonal antibody in algal chloroplasts

Tran, M., Zhou, B., Pettersson, P. L., González, M. J., & Mayfield, S. P. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22446 · Cited by 183 · see citing works

Enzymatic cell disruption of the microalgae Chlamydomonas reinhardtii for lipid and protein extraction

Sierra, L. S., Dixon, C. K., & Wilken, L. R. (2017) · Algal Research · doi:10.1016/j.algal.2017.04.004 · Cited by 176 · see citing works

Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects

Gong, Y., Hu, H., Gao, Y., Xu, X., & Gao, H. C. (2011) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-011-1032-6 · Cited by 168 · see citing works

Molecular techniques to interrogate and edit the Chlamydomonas nuclear genome

Jinkerson, R. E., & Jonikas, M. C. (2015) · The Plant Journal · doi:10.1111/tpj.12801 · Cited by 162 · see citing works

Algae-based oral recombinant vaccines

Specht, E. A., & Mayfield, S. P. (2014) · Frontiers in Microbiology · doi:10.3389/fmicb.2014.00060 · Cited by 151 · see citing works

Biotechnological exploitation of microalgae

Gangl, D., Zedler, J. A. Z., Rajakumar, P. D., Ramos‐Martinez, E. M., Riseley, A. S., Włodarczyk, A., Purton, S., Sakuragi, Y., Howe, C. J., Jensen, P. E., et al. (2015) · Journal of Experimental Botany · doi:10.1093/jxb/erv426 · Cited by 145 · see citing works

Expression and characterization of antimicrobial peptides Retrocyclin‐101 and Protegrin‐1 in chloroplasts to control viral and bacterial infections

Lee, S.-B., Li, B., Jin, S., & Daniell, H. (2010) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2010.00538.x · Cited by 141 · see citing works

The algal chloroplast as a synthetic biology platform for production of therapeutic proteins

Dyo, Y. M., & Purton, S. (2018) · Microbiology · doi:10.1099/mic.0.000599 · Cited by 140 · see citing works

Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission

Gregory, J. A., Li, F., Tomosada, L. M., Cox, C., Topol, A. B., Vinetz, J. M., & Mayfield, S. P. (2012) · PLoS ONE · doi:10.1371/journal.pone.0037179 · Cited by 139 · see citing works

Expanding the spectral palette of fluorescent proteins for the green microalga C hlamydomonas reinhardtii

Rasala, B. A., Barrera, D. J., Ng, J. Y., Plucinak, T. M., Rosenberg, J. N., Weeks, D. P., Oyler, G. A., Peterson, T. C., Haerizadeh, F., & Mayfield, S. P. (2013) · The Plant Journal · doi:10.1111/tpj.12165 · Cited by 137 · see citing works

Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives

Rosales‐Mendoza, S., Paz-Maldonado, L. M. T., & Soria‐Guerra, R. E. (2011) · Plant Cell Reports · doi:10.1007/s00299-011-1186-8 · Cited by 133 · see citing works

Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines

Gregory, J. A., Topol, A. B., Doerner, D. Z., & Mayfield, S. P. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.00714-13 · Cited by 132 · see citing works

The microalga<i>Chlamydomonas reinhardtii</i>as a platform for the production of human protein therapeutics

Rasala, B. A., & Mayfield, S. P. (2011) · Bioengineered Bugs · doi:10.4161/bbug.2.1.13423 · Cited by 122 · see citing works

New tools for chloroplast genetic engineering allow the synthesis of human growth hormone in the green alga Chlamydomonas reinhardtii

Brocklehurst, T. W., Waterhouse, J. C., Young, R., Economou, C. K., & Purton, S. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7354-6 · Cited by 116 · see citing works

Industrial Use of Cell Wall Degrading Enzymes: The Fine Line Between Production Strategy and Economic Feasibility

Giovannoni, M., Gramegna, G., Benedetti, M., & Mattei, B. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00356 · Cited by 110 · see citing works

Gene Flow, Risk Assessment and the Environmental Release of Transgenic Plants

Chandler, S. F., & Dunwell, J. M. (2008) · Critical Reviews in Plant Sciences · doi:10.1080/07352680802053916 · Cited by 110 · see citing works

Enhanced chloroplast transgene expression in a nuclear mutant of Chlamydomonas

Michelet, L., Lefebvre‐Legendre, L., Burr, S. E., Rochaix, J., & Goldschmidt‐Clermont, M. (2010) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2010.00564.x · Cited by 110 · see citing works

Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii

Lauersen, K. J., Berger, H., Mussgnug, J. H., & Kruse, O. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.10.010 · Cited by 107 · see citing works

Advances and challenges in genetic engineering of microalgae

Fajardo, C., Donato, M. D., Carrasco-Reinado, R., Martı́nez-Rodrı́guez, G., Mancera, J. M., & Fernández‐Acero, F. J. (2019) · Reviews in Aquaculture · doi:10.1111/raq.12322 · Cited by 103 · see citing works

Recent achievements obtained by chloroplast transformation

Adem, M., Beyene, D., & Feyissa, T. (2017) · Plant Methods · doi:10.1186/s13007-017-0179-1 · Cited by 102 · see citing works

Chlamydomonas reinhardtii: A Factory of Nutraceutical and Food Supplements for Human Health

Masi, A., Leonelli, F., Scognamiglio, V., Gasperuzzo, G., Antonacci, A., & Terzidis, M. A. (2023) · Molecules · doi:10.3390/molecules28031185 · Cited by 96 · see citing works

Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5′ untranslated region optimization

Rasala, B. A., Muto, M., Sullivan, J., & Mayfield, S. P. (2011) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2011.00620.x · Cited by 97 · see citing works

Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii

Jones, C. S., Luong, T. Q., Hannon, M., Tran, M., Gregory, J. A., Shen, Z., Briggs, S. P., & Mayfield, S. P. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4071-7 · Cited by 93 · see citing works

A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)

Wang, X., Brandsma, M. E., Tremblay, R., Maxwell, D. P., Jevnikar, A. M., Hüner, N. P. A., & Ma, S. (2008) · BMC Biotechnology · doi:10.1186/1472-6750-8-87 · Cited by 93 · see citing works

Synthetic promoters capable of driving robust nuclear gene expression in the green alga Chlamydomonas reinhardtii

Scranton, M. A., Ostrand, J. T., Georgianna, D. R., Lofgren, S., Li, D. D., Ellis, R. C., Carruthers, D. N., Dräger, A., Masica, D. L., & Mayfield, S. P. (2016) · Algal Research · doi:10.1016/j.algal.2016.02.011 · Cited by 93 · see citing works

Production of recombinant proteins in microalgae at pilot greenhouse scale

Gimpel, J. A., Hyun, J. S., Schoepp, N. G., & Mayfield, S. P. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25357 · Cited by 86 · see citing works

Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?

Yu, Y., Yu, P.-C., Chang, W., Yu, K., & Lin, C. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21144854 · Cited by 85 · see citing works

Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

Almaraz-Delgado, A. L., Flores‐Uribe, J., Pérez-España, V. H., Salgado, E., & Badillo-Corona, J. A. (2014) · AMB Express · doi:10.1186/s13568-014-0057-4 · Cited by 82 · see citing works

Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum

Xie, W., Zhu, C.-C., Zhang, N., Li, D., Yang, W., Liu, J., Ramalingam, S., & Li, H. (2014) · Marine Biotechnology · doi:10.1007/s10126-014-9570-3 · Cited by 82 · see citing works

Application of the CRISPR/Cas system for genome editing in microalgae

Zhang, Y., Jiang, J., Shi, T., Sun, X., Zhao, Q., Huang, H., & Ren, L. (2019) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-09726-x · Cited by 80 · see citing works

Production of anti‐cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners

Tran, M., Henry, R. E., Siefker, D. T., Van, C., Newkirk, G. M., Kim, J. J., Bui, J. D., & Mayfield, S. P. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.24966 · Cited by 79 · see citing works

Biological potential of microalgae in China for biorefinery-based production of biofuels and high value compounds

Li, J., Liu, Y., Cheng, J. J., Mos, M., & Daroch, M. (2015) · New Biotechnology · doi:10.1016/j.nbt.2015.02.001 · Cited by 79 · see citing works

Microalgae, Functional Genomics and Biotechnology

Cadoret, J., Garnier, M., & Saint‐Jean, B. (2012) · Advances in botanical research · doi:10.1016/b978-0-12-391499-6.00008-6 · Cited by 79 · see citing works

Plant-derived vaccines and other therapeutics produced in contained systems

Franconi, R., Demurtas, O. C., & Massa, S. (2010) · Expert Review of Vaccines · doi:10.1586/erv.10.91 · Cited by 78 · see citing works

Perspectives for Glyco-Engineering of Recombinant Biopharmaceuticals from Microalgae

Barolo, L., Abbriano, R. M., Commault, A. S., George, J., Kahlke, T., Fabris, M., Padula, M. P., Lopez, A., Ralph, P. J., & Pernice, M. (2020) · Cells · doi:10.3390/cells9030633 · Cited by 65 · see citing works

Fed-batch mixotrophic cultivation of Chlamydomonas reinhardtii for high-density cultures

Fields, F. J., Ostrand, J. T., & Mayfield, S. P. (2018) · Algal Research · doi:10.1016/j.algal.2018.05.006 · Cited by 66 · see citing works

Towards developing algal synthetic biology

Scaife, M. A., & Smith, A. G. (2016) · Biochemical Society Transactions · doi:10.1042/bst20160061 · Cited by 64 · see citing works

Therapeutic proteins: developments, progress, challenges, and future perspectives

Kumar, V., Barwal, A., Sharma, N., Mir, D. S., Kumar, P., & Kumar, V. (2024) · 3 Biotech · doi:10.1007/s13205-024-03958-z · Cited by 62 · see citing works

Harnessing genetic engineering to drive economic bioproduct production in algae

Gupta, A., Kang, K., Pathania, R., Saxton, L., Saucedo, B., Malik, A., Torres-Tiji, Y., Diaz, C. J., Dutra Molino, J. V., & Mayfield, S. P. (2024) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2024.1350722 · Cited by 60 · see citing works

Evaluating nuclear transgene expression systems in Chlamydomonas reinhardtii

Kumar, A., Falcão, V. D. R., & Sayre, R. T. (2013) · Algal Research · doi:10.1016/j.algal.2013.09.002 · Cited by 59 · see citing works

Microalgae as Solar-Powered Protein Factories

Hempel, F., & Maier, U. G. (2016) · Advances in experimental medicine and biology · doi:10.1007/978-3-319-27216-0_16 · Cited by 57 · see citing works

Algal chloroplast produced camelid V H H antitoxins are capable of neutralizing botulinum neurotoxin

Barrera, D. J., Rosenberg, J. N., Chiu, J. G., Chang, Y., Debatis, M. E., Ngoi, S., Chang, J. T., Shoemaker, C. B., Oyler, G. A., & Mayfield, S. P. (2014) · Plant Biotechnology Journal · doi:10.1111/pbi.12244 · Cited by 56 · see citing works

Synthetic biology approaches for the production of plant metabolites in unicellular organisms

Moses, T., Mehrshahi, P., Smith, A. G., & Goossens, A. (2017) · Journal of Experimental Botany · doi:10.1093/jxb/erx119 · Cited by 55 · see citing works

Constitutive and Chloroplast Targeted Expression of Acetyl-CoA Carboxylase in Oleaginous Microalgae Elevates Fatty Acid Biosynthesis

Li, D., Xie, W., Hao, T.-B., Cai, J.-X., Zhou, T., Balamurugan, S., Yang, W., Liu, J., & Li, H. (2018) · Marine Biotechnology · doi:10.1007/s10126-018-9841-5 · Cited by 55 · see citing works

An evaluation of microalgae as a recombinant protein oral delivery platform for fish using green fluorescent protein (GFP)

Kwon, K., Lamb, A., Fox, D., & Jegathese, S. J. P. (2019) · Fish & Shellfish Immunology · doi:10.1016/j.fsi.2019.01.038 · Cited by 53 · see citing works

Synthesis of bacteriophage lytic proteins against Streptococcus pneumoniae in the chloroplast of Chlamydomonas reinhardtii

Stoffels, L., Taunt, H. N., Charalambous, B. M., & Purton, S. (2017) · Plant Biotechnology Journal · doi:10.1111/pbi.12703 · Cited by 50 · see citing works

A New Strategy to Produce a Defensin: Stable Production of Mutated NP-1 in Nitrate Reductase-Deficient Chlorella ellipsoidea

Bai, L.-L., Yin, W.-B., Chen, Y., Niu, L., Sun, Y., Zhao, S., Yang, F., Wang, R. R. ‐., Wu, Q., Zhang, X., et al. (2013) · PLoS ONE · doi:10.1371/journal.pone.0054966 · Cited by 48 · see citing works

High‐value Recombinant Protein Production in Microalgae

Barrera, D. J., & Mayfield, S. P. (2013) · doi:10.1002/9781118567166.ch27 · Cited by 48 · see citing works

The phosphite oxidoreductase gene, ptxD as a bio-contained chloroplast marker and crop-protection tool for algal biotechnology using Chlamydomonas

Changko, S., Rajakumar, P. D., Young, R., & Purton, S. (2019) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-019-10258-7 · Cited by 44 · see citing works

Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast

Muto, M., Henry, R. E., & Mayfield, S. P. (2009) · BMC Biotechnology · doi:10.1186/1472-6750-9-26 · Cited by 43 · see citing works

Harnessing the Algal Chloroplast for Heterologous Protein Production

Cutolo, E. A., Mandalà, G., Dall’Osto, L., & Bassi, R. (2022) · Microorganisms · doi:10.3390/microorganisms10040743 · Cited by 43 · see citing works

Recent Advances in Marine Microalgae Production: Highlighting Human Health Products from Microalgae in View of the Coronavirus Pandemic (COVID-19)

Ashour, M., & Omran, A. M. M. (2022) · Fermentation · doi:10.3390/fermentation8090466 · Cited by 40 · see citing works

Natural Variants of Photosystem II Subunit D1 Tune Photochemical Fitness to Solar Intensity *

Vinyard, D. J., Gimpel, J. A., Ananyev, G., Cornejo, M. A., Golden, S. S., Mayfield, S. P., & Dismukes, G. C. (2012) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.394668 · Cited by 40 · see citing works

Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast

Ma, K., Deng, L., Wu, H., & Fan, J. (2022) · Bioresources and Bioprocessing · doi:10.1186/s40643-022-00568-6 · Cited by 39 · see citing works

Engineered Photosystem II Reaction Centers Optimize Photochemistry versus Photoprotection at Different Solar Intensities

Vinyard, D. J., Gimpel, J. A., Ananyev, G., Mayfield, S. P., & Dismukes, G. C. (2014) · Journal of the American Chemical Society · doi:10.1021/ja5002967 · Cited by 38 · see citing works

Processing Methodologies of Wet Microalga Biomass Toward Oil Separation: An Overview

Pôjo, V., Tavares, T. G., & Malcata, F. X. (2021) · Molecules · doi:10.3390/molecules26030641 · Cited by 38 · see citing works

Microalgae-made vaccines against infectious diseases

Ramos‐Vega, A., Angulo, C., Bañuelos-Hernández, B., & Monreal‐Escalante, E. (2021) · Algal Research · doi:10.1016/j.algal.2021.102408 · Cited by 38 · see citing works

Development of a constitutive and an auto-inducible high-yield expression system for recombinant protein production in the microalga Nannochloropsis oceanica

de Grahl, I., Rout, S. S., Maple‐Grødem, J., & Reumann, S. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10789-4 · Cited by 35 · see citing works

Nuclear genome shuffling significantly increases production of chloroplast-based recombinant protein in Chlamydomonas reinhardtii

Fields, F. J., Ostrand, J. T., Tran, M., & Mayfield, S. P. (2019) · Algal Research · doi:10.1016/j.algal.2019.101523 · Cited by 33 · see citing works

A Review of the Sustainability, Chemical Composition, Bioactive Compounds, Antioxidant and Antidiabetic Activity, Neuroprotective Properties, and Health Benefits of Microalgae

Dimopoulou, M., Kolonas, A., Stagos, D., & Gortzi, O. (2025) · Biomass · doi:10.3390/biomass5010011 · Cited by 34 · see citing works

Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory

Kwon, Y. M., Kim, K. W., Choi, T. Y., Kim, S. Y., & Kim, J. Y. H. (2018) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-018-2567-8 · Cited by 31 · see citing works

Overcoming Intrinsic Restriction Enzyme Barriers Enhances Transformation Efficiency in Arthrospira platensis C1

Jeamton, W., Dulsawat, S., Tanticharoen, M., Vonshak, A., & Cheevadhanarak, S. (2017) · Plant and Cell Physiology · doi:10.1093/pcp/pcx016 · Cited by 31 · see citing works

Liquid culture of microalgae in a photobioreactor (PBR) based on oscillatory baffled reactor (OBR) technology – A feasibility study

Abbott, M. S. R., Brain, C. M., Harvey, A., Morrison, M. I., & Perez, G. V. (2015) · Chemical Engineering Science · doi:10.1016/j.ces.2015.07.045 · Cited by 29 · see citing works

Method to assemble and integrate biochemical pathways into the chloroplast genome of Chlamydomonas reinhardtii

Noor‐Mohammadi, S., Pourmir, A., & Johannes, T. W. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24569 · Cited by 29 · see citing works

Improved high-throughput screening technique to rapidly isolate Chlamydomonas transformants expressing recombinant proteins

Sproles, A. E., Berndt, A., Fields, F. J., & Mayfield, S. P. (2022) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-022-11790-9 · Cited by 28 · see citing works

Analysis of heterologous regulatory and coding regions in algal chloroplasts

Gimpel, J. A., & Mayfield, S. P. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4580-4 · Cited by 29 · see citing works

An Insight into the Algal Evolution and Genomics

Khan, A. K., Kausar, H., Jaferi, S. S., Drouet, S., Hano, C., Abbasi, B., & Anjum, S. (2020) · Biomolecules · doi:10.3390/biom10111524 · Cited by 18 · see citing works

Production of Recombinant Biopharmaceuticals in Chlamydomonas reinhardtii

Arias, C. A. D., de Oliveira, C. F. M., Molino, J. V. D., Ferreira‐Camargo, L. S., Matsudo, M. C., & de Carvalho, J. C. M. (2022) · International Journal of Plant Biology · doi:10.3390/ijpb14010004 · Cited by 24 · see citing works

An inducible expression system in the alga Nannochloropsis gaditana controlled by the nitrate reductase promoter

Jackson, H. O., Berepiki, A., Baylay, A. J., Terry, M. J., Moore, C. M., & Bibby, T. S. (2018) · Journal of Applied Phycology · doi:10.1007/s10811-018-1510-6 · Cited by 23 · see citing works

Genetic Engineering to Improve Algal Biofuels Production

Rasala, B. A., Gimpel, J. A., Tran, M., Hannon, M. J., Miyake-Stoner, S. J., Specht, E. A., & Mayfield, S. P. (2012) · Springer eBooks · doi:10.1007/978-94-007-5479-9_6 · Cited by 24 · see citing works

Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii

Gimpel, J. A., Nour‐Eldin, H. H., Scranton, M. A., Li, D. D., & Mayfield, S. P. (2015) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00076 · Cited by 23 · see citing works

An improved ARS2‐derived nuclear reporter enhances the efficiency and ease of genetic engineering in Chlamydomonas

Specht, E. A., Nour‐Eldin, H. H., Hoang, K. T. D., & Mayfield, S. P. (2014) · Biotechnology Journal · doi:10.1002/biot.201400172 · Cited by 22 · see citing works

The potential of transgenic green microalgae; a robust photobioreactor to produce recombinant therapeutic proteins

Akbari, F., Eskandani, M., & Khosroushahi, A. Y. (2014) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-014-1714-0 · Cited by 22 · see citing works

Les microalgues

Cadoret, J., Bardor, M., Lerouge, P., Cabigliera, M., Henríquez, V., & Carlier, A. (2008) · médecine/sciences · doi:10.1051/medsci/2008244375 · Cited by 22 · see citing works

Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii

Campos-Quevedo, N., Rosales‐Mendoza, S., Paz-Maldonado, L. M. T., Martínez-Salgado, L., Guevara-Arauza, J. C., & Soria‐Guerra, R. E. (2012) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-012-0261-3 · Cited by 22 · see citing works

Genetic Engineering for Microalgae Strain Improvement in Relation to Biocrude Production Systems

Stephens, E., Wolf, J., Oey, M., Zhang, E., Hankamer, B. D., & Ross, I. L. (2015) · Biofuel and biorefinery technologies · doi:10.1007/978-3-319-16640-7_11 · Cited by 22 · see citing works

A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts

Richter, L. V., Yang, H., Yazdani, M., Hanson, M. R., & Ahner, B. A. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1127-7 · Cited by 22 · see citing works

Algae: An Alternative to the Higher Plant System in Gene Farming

Griesbeck, C., & Kirchmayr, A. (2011) · Springer eBooks · doi:10.1007/978-94-007-2217-0_6 · Cited by 21 · see citing works

Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii

Rasala, B. A., Muto, M., Lee, P. A., Jager, M., Cardoso, R. M. F., Behnke, C. A., Kirk, P. B., Hokanson, C. A., Crea, R., Mendez, M. J., et al. (2010) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2010.00503.x · Cited by 271 · see citing works

Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii

Almaraz-Delgado, A. L., Flores‐Uribe, J., Pérez-España, V. H., Salgado, E., & Badillo-Corona, J. A. (2014) · AMB Express · doi:10.1186/s13568-014-0057-4 · Cited by 82 · see citing works

Optimization of Recombinant Protein Expression in the Chloroplasts of Green Algae

Fletcher, S. P., Muto, M., & Mayfield, S. P. (2008) · Advances in experimental medicine and biology · doi:10.1007/978-0-387-75532-8_8 · Cited by 39 · see citing works

A rapid, modular and marker-free chloroplast expression system for the green alga Chlamydomonas reinhardtii

Bertalan, I., Munder, M. C., Weiß, C., Kopf, J., Fischer, D., & Johanningmeier, U. (2014) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2014.12.017 · Cited by 49 · see citing works

Production of recombinant proteins in microalgae at pilot greenhouse scale

Gimpel, J. A., Hyun, J. S., Schoepp, N. G., & Mayfield, S. P. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25357 · Cited by 86 · see citing works