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The crystal structure of urease from Klebsiella aerogenes
Abstract
The crystal structure of urease from Klebsiella aerogenes has been determined at 2.2 A resolution and refined to an R factor of 18.2 percent. The enzyme contains four structural domains: three with novel folds playing structural roles, and an (alpha beta)8 barrel domain, which contains the bi-nickel center. The two active site nickels are 3.5 A apart. One nickel ion is coordinated by three ligands (with low occupancy of a fourth ligand) and the second is coordinated by five ligands. A carbamylated lysine provides an oxygen ligand to each nickel, explaining why carbon dioxide is required for the activation of urease apoenzyme. The structure is compatible with a catalytic mechanism whereby urea ligates Ni-1 to complete its tetrahedral coordination and a hydroxide ligand of Ni-2 attacks the carbonyl carbon. A surprisingly high structural similarity between the urease catalytic domain and that of the zinc-dependent adenosine deaminase reveals a remarkable example of active site divergence.
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Kabsch, W., & Sander, C. (1983) · Biopolymers · doi:10.1002/bip.360221211 · Cited by 15,320
Protein Structure Comparison by Alignment of Distance Matrices
Holm, L., & Sander, C. (1993) · Journal of Molecular Biology · doi:10.1006/jmbi.1993.1489 · Cited by 3,984
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Metadata
- Authors
- Jabri, E., Carr, M., Hausinger, R., & Karplus, P.
- Venue
- Science
- Published
- 1995-05-19
- Volume
- 268
- Issue
- 5213
- Pages
- 998-1004
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2015304573
- Cited by
- 830
- References
- 2
- FWCI
- 34.08
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jabri, E., Carr, M., Hausinger, R., & Karplus, P. (1995). The crystal structure of urease from Klebsiella aerogenes. Science. https://doi.org/10.1126/science.7754395
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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