The DOI matches its Crossref record.
Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein
Abstract
The RAD51 gene of Saccharomyces cerevisiae is required for genetic recombination and DNA double-strand break repair. Here it is demonstrated that RAD51 protein pairs circular viral single-stranded DNA from φX 174 or M13 with its respective homologous linear double-stranded form. The product of synapsis between these DNA partners is further processed by RAD51 to yield nicked circular duplex DNA, which indicates that RAD51 can catalyze strand exchange. The pairing and strand exchange reaction requires adenosine triphosphate, a result consistent with the presence of a DNA-dependent adenosine triphosphatase activity in RAD51 protein. Thus, RAD51 is a eukaryotic recombination protein that can catalyze the strand exchange reaction.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
No references could be resolved yet.
Research works printed but not linked: 19
- ABOUSSEKHRA, A, SEMIDOMINANT SUPPRESSORS OF SRS2 HELICASE MUTATIONS OF SACCHAROMYCES-CEREVISIAE MAP IN THE RAD51 GENE, WHOSE SEQUENCE PREDICTS A PROTEIN WITH SIMILARITIES TO PROKARYOTIC RECA PROTEINS, MOLECULAR AND CELLULAR BIOLOGY 12: 3224 (1992).
- BASILE, G, NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE YEAST RECOMBINATIONAL REPAIR GENE RAD51, MOLECULAR AND CELLULAR BIOLOGY 12: 3235 (1992).
- BEZZUBOVA, O, A CHICKEN RAD51 HOMOLOG IS EXPRESSED AT HIGH-LEVELS IN LYMPHOID AND REPRODUCTIVE-ORGANS, NUCLEIC ACIDS RESEARCH 21: 1577 (1993). · DOI
- 10.1016/0092-8674(92)90446-J · DOI · Paper page
- BRILL, S.J., YEAST REPLICATION FACTOR-A FUNCTIONS IN THE UNWINDING OF THE SV40 ORIGIN OF DNA-REPLICATION, NATURE 342: 92 (1989). · DOI · Paper page
- GUZDER, S.N., RAD25 IS A DNA HELICASE REQUIRED FOR RNA REPAIR AND RNA-POLYMERASE-II TRANSCRIPTION, NATURE 369: 578 (1994). · DOI · Paper page
- KANS, J.A., NUCLEOTIDE-SEQUENCE OF THE RAD57 GENE OF SACCHAROMYCES-CEREVISIAE, GENE 105: 139 (1991). · DOI
- KLECKNER, N, MEIOTIC CHROMOSOME METABOLISM - ONE VIEW, COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY 56: 729 (1991). · DOI
- MORITA, T, A MOUSE HOMOLOG OF THE ESCHERICHIA-COLI RECA AND SACCHAROMYCES-CEREVISIAE RAD51 GENES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 90: 6577 (1993).
- 10.1126/science.8456314 · DOI · Paper page
- Petes, T. D., The Molecular and Cellular Biology of the Yeast Saccharomyces: Genome Dynamics, Protein Synthesis, and Energetics: 407 (1991).
- RADDING, C.M., HELICAL INTERACTIONS IN HOMOLOGOUS PAIRING AND STRAND EXCHANGE DRIVEN BY RECA PROTEIN, JOURNAL OF BIOLOGICAL CHEMISTRY 266: 5355 (1991). · DOI · Paper page
- ROCA, A.I., THE RECA PROTEIN - STRUCTURE AND FUNCTION, CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY 25: 415 (1990). · DOI · Paper page
- SHINOHARA, A, RAD51 PROTEIN INVOLVED IN REPAIR AND RECOMBINATION IN SACCHAROMYCES-CEREVISIAE IS A RECA-LIKE PROTEIN, CELL 69: 457 (1992). · DOI · Paper page
- SHINOHARA, A, CLONING OF HUMAN, MOUSE AND FISSION YEAST RECOMBINATION GENES HOMOLOGOUS TO RAD51 AND RECA, NATURE GENETICS 4: 239 (1993). · DOI · Paper page
- STORY, R.M., STRUCTURAL RELATIONSHIP OF BACTERIAL RECA PROTEINS TO RECOMBINATION PROTEINS FROM BACTERIOPHAGE-T4 AND YEAST, SCIENCE 259: 1892 (1993). · DOI
- SUNG, P, HUMAN XERODERMA-PIGMENTOSUM GROUP-D GENE ENCODES A DNA HELICASE, NATURE 365: 852 (1993). · DOI · Paper page
- SUNG, P, THE RAD3 GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A DNA-DEPENDENT ATPASE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 6045 (1987).
- YOSHIMURA, Y, CLONING AND SEQUENCE OF THE HUMAN RECA-LIKE GENE CDNA, NUCLEIC ACIDS RESEARCH 21: 1665 (1993). · DOI
Cited by
Molecular Mechanisms of Mammalian DNA Repair and the DNA Damage Checkpoints
Sancar, A., Lindsey-Boltz, L. A., Ünsal-Kaçmaz, K., & Linn, S. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073723 · Cited by 3,317
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Pâques, F., & Haber, J. E. (1999) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.63.2.349-404.1999 · Cited by 2,120
Association of BRCA1 with Rad51 in Mitotic and Meiotic Cells
Scully, R., Chen, J., Plug, A., Xiao, Y., Weaver, D., Feunteun, J., Ashley, T., & Livingston, D. M. (1997) · Cell · doi:10.1016/s0092-8674(00)81847-4 · Cited by 1,459
XRCC3 promotes homology-directed repair of DNA damage in mammalian cells
Pierce, A. J., Johnson, R. D., Thompson, L. H., & Jasin, M. (1999) · Genes & Development · doi:10.1101/gad.13.20.2633 · Cited by 1,379
Yoshida, K., & Miki, Y. (2004) · Cancer Science · doi:10.1111/j.1349-7006.2004.tb02195.x · Cited by 753
Metadata
- Authors
- Sung, P.
- Venue
- Science
- Published
- 1994-08-26
- Volume
- 265
- Issue
- 5176
- Pages
- 1241-1243
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2052521064
- Cited by
- 866
- References
- 0
- FWCI
- 15.20
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein. https://app.cochat.ai/papers/10.1126/science.8066464
- The paper
- Sung, P. (1994). Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein. Science. https://doi.org/10.1126/science.8066464
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
