The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Protein Ladder Sequencing
Abstract
A new approach to protein sequencing is described. It consists of two steps: (i) ladder-generating chemistry, the controlled generation from a polypeptide chain by wet chemistry of a family of sequence-defining peptide fragments, each differing from the next by one amino acid; and (ii) data readout, a one-step readout of the resulting protein sequencing ladder by matrix-assisted laser-desorption mass spectrometry. Each amino acid was identified from the mass difference between successive peaks, and the position in the data set defined the sequence of the original peptide chain. This method was used to directly locate a phosphoserine residue in a phosphopeptide. The protein ladder sequencing method lends itself to very high sample throughput at very low per cycle cost.
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Citations
Cites
Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers.
Hillenkamp, F., Karas, M., Beavis, R. C., & Chait, B. T. (1991) · Analytical Chemistry · doi:10.1021/ac00024a716 · Cited by 1,930
Self-citation
Cited by
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Wyttenbach, T., von Helden, G., & Bowers, M. T. (1996) · Journal of the American Chemical Society · doi:10.1021/ja9535928 · Cited by 384
Veerendra Kumar, M. H., & Gupta, Y. K. (2002) · Journal of Ethnopharmacology · doi:10.1016/s0378-8741(01)00394-4 · Cited by 261
Antioxidant property of Celastrus paniculatus Willd.: A possible mechanism in enhancing cognition
Kumar, M. H. V., & Gupta, Y. K. (2002) · Phytomedicine · doi:10.1078/0944-7113-00136 · Cited by 133
Metadata
- Authors
- Chait, B. T., Wang, R., Beavis, R. C., & Kent, S. B. H.
- Venue
- Science
- Published
- 1993-10
- Volume
- 262
- Issue
- 5130
- Pages
- 89-92
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2018145486
- Cited by
- 309
- References
- 1
- FWCI
- 20.53
- Type
- journal article
- Language
- en
- Open access
- closed
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- Chait, B. T., Wang, R., Beavis, R. C., & Kent, S. B. H. (1993). Protein Ladder Sequencing. Science. https://doi.org/10.1126/science.8211132
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
