The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structure at 2.5 Å of a Designed Peptide that Maintains Solubility of Membrane Proteins
Abstract
A 24-amino acid peptide designed to solubilize integral membrane proteins has been synthesized. The design was for an amphipathic α helix with a "flat" hydrophobic surface that would interact with a transmembrane protein as a detergent. When mixed with peptide, 85 percent of bacteriorhodopsin and 60 percent of rhodopsin remained in solution over a period of 2 days in their native forms. The crystal structure of peptide alone showed it to form an antiparallel four-helix bundle in which monomers interact, flat surface to flat surface, as predicted.
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Citations
Cites
The packing of α-helices: simple coiled-coils
Crick, F. H. C. (1953) · Acta Crystallographica · doi:10.1107/s0365110x53001964 · Cited by 1,578
Cited by
Detergents for the stabilization and crystallization of membrane proteins
Privé, G. G. (2007) · Methods · doi:10.1016/j.ymeth.2007.01.007 · Cited by 532
Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions
Tribet, C., Audebert, R., & Popot, J.-L. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.26.15047 · Cited by 473
Engineering Escherichia coli for production of functionalized terpenoids using plant P450s
Chang, M. C. Y., Eachus, R. A., Trieu, W., Ro, D.-K., & Keasling, J. D. (2007) · Nature Chemical Biology · doi:10.1038/nchembio875 · Cited by 364
A saposin-lipoprotein nanoparticle system for membrane proteins
Frauenfeld, J., Löving, R., Armache, J.-P., Sonnen, A. F.-P., Guettou, F., Moberg, P., Zhu, L., Jegerschöld, C., Flayhan, A., Briggs, J. A. G., et al. (2016) · Nature Methods · doi:10.1038/nmeth.3801 · Cited by 293
Engineering of soluble bacteriorhodopsin
Nikolaev, A., Orlov, Y., Tsybrov, F., Kuznetsova, E., Shishkin, P., Kuzmin, A., Mikhailov, A., Nikolaeva, Y. S., Anuchina, A., Chizhov, I., et al. (2025) · Chemical Science · doi:10.1039/d5sc02453f · Cited by 6
Metadata
- Authors
- Schafmeister, C. E., Miercke, L. J. W., & Stroud, R. M.
- Venue
- Science
- Published
- 1993-10-29
- Volume
- 262
- Issue
- 5134
- Pages
- 734-738
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2079148256
- Cited by
- 201
- References
- 1
- FWCI
- 7.24
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Structure at 2.5 Å of a Designed Peptide that Maintains Solubility of Membrane Proteins. https://app.cochat.ai/papers/10.1126/science.8235592
- The paper
- Schafmeister, C. E., Miercke, L. J. W., & Stroud, R. M. (1993). Structure at 2.5 Å of a Designed Peptide that Maintains Solubility of Membrane Proteins. Science. https://doi.org/10.1126/science.8235592
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
