The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
A saposin-lipoprotein nanoparticle system for membrane proteins
Open accessAbstract
A limiting factor in membrane protein research is the ability to solubilize and stabilize such proteins. Detergents are used most often for solubilizing membrane proteins, but they are associated with protein instability and poor compatibility with structural and biophysical studies. Here we present a saposin-lipoprotein nanoparticle system, Salipro, which allows for the reconstitution of membrane proteins in a lipid environment that is stabilized by a scaffold of saposin proteins. We demonstrate the applicability of the method on two purified membrane protein complexes as well as by the direct solubilization and nanoparticle incorporation of a viral membrane protein complex from the virus membrane. Our approach facilitated high-resolution structural studies of the bacterial peptide transporter PeptTSo2 by single-particle cryo-electron microscopy (cryo-EM) and allowed us to stabilize the HIV envelope glycoprotein in a functional state.
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Self-citationFusion protein strategies for cryo-EM study of G protein-coupled receptors
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Self-citationStructural insights into binding-site access and ligand recognition by human ABCB1
Kurre, D., Dang, P. X., Le, L. T. M., Gadkari, V. V., & Alam, A. (2025) · The EMBO Journal · doi:10.1038/s44318-025-00361-z · Cited by 25
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Metadata
- Authors
- Frauenfeld, J., Löving, R., Armache, J.-P., Sonnen, A. F.-P., Guettou, F., Moberg, P., Zhu, L., Jegerschöld, C., Flayhan, A., Briggs, J. A. G., Garoff, H., Löw, C., Cheng, Y., & Nordlund, P.
- Venue
- Nature Methods
- Published
- 2016-03-07
- Volume
- 13
- Issue
- 4
- Pages
- 345-351
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2294803096
- Cited by
- 293
- References
- 51
- FWCI
- 11.14
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Frauenfeld, J., Löving, R., Armache, J.-P., Sonnen, A. F.-P., Guettou, F., Moberg, P., Zhu, L., Jegerschöld, C., Flayhan, A., Briggs, J. A. G., Garoff, H., Löw, C., Cheng, Y., & Nordlund, P. (2016). A saposin-lipoprotein nanoparticle system for membrane proteins. Nature Methods. https://doi.org/10.1038/nmeth.3801
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