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Field-Flow Fractionation: Analysis of Macromolecular, Colloidal, and Particulate Materials
Abstract
Field-flow fractionation (FFF) is a family of flexible elution techniques capable of simultaneous separation and measurement. Its sample domain extends across a broad macromolecular-colloidal-particulate continuum from about 1 nanometer to more than 100 micrometers and incorporates both simple and complex macromaterials of biological, biomedical, industrial, and environmental relevance. Complex materials are separated into components to simplify measurement. Component properties measurable by FFF include mass, size, density, charge, diffusivity, and thickness of adsorbed layers. When characterization by these properties is inadequate, other measurement tools can be readily coupled to FFF, either off-line or on-line, by virtue of its flow-elution operation. This article describes the principles and major subtechniques of the FFF family along with application of its measurement and separative capabilities.
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Cited by
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Yamada, M., Nakashima, M., & Seki, M. (2004) · Analytical Chemistry · doi:10.1021/ac049863r · Cited by 746
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Schwaferts, C., Niessner, R., Elsner, M., & Ivleva, N. P. (2019) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2018.12.014 · Cited by 522
Inertial microfluidics for continuous particle filtration and extraction
Bhagat, A. A. S., Kuntaegowdanahalli, S. S., & Papautsky, I. (2009) · Microfluidics and Nanofluidics · doi:10.1007/s10404-008-0377-2 · Cited by 334
Chen, Y., Guo, Z., Wang, X., & Qiu, C. (2008) · Journal of Chromatography A · doi:10.1016/j.chroma.2007.10.026 · Cited by 316
Metadata
- Authors
- Giddings, J. C.
- Venue
- Science
- Published
- 1993-06-04
- Volume
- 260
- Issue
- 5113
- Pages
- 1456-1465
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2103633180
- Cited by
- 1,141
- References
- 0
- FWCI
- 25.12
- Type
- journal article
- Language
- en
- Open access
- closed
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- Giddings, J. C. (1993). Field-Flow Fractionation: Analysis of Macromolecular, Colloidal, and Particulate Materials. Science. https://doi.org/10.1126/science.8502990
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