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Cited by 278HIV Research and TreatmentRNA and protein synthesis mechanismsDNA and Nucleic Acid Chemistry

Structure of the HIV-1 RNA packaging signal

Open access
Keane, Sarah C., Heng, Xiao, Lu, Kun, Kharytonchyk, Siarhei, Ramakrishnan, Venkateswaran, Carter, Gregory, Barton, Shawn, Hosic, Azra +9 more·2015·Science
DOI 10.1126/science.aaa9266PMC PMC4492308
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Abstract

Structural signals that direct HIV packaging: During the viral replication cycle of HIV, unspliced dimeric RNA genomes are efficiently packaged into new virions at the host cell membrane. Packaging is directed by a region at the start of the genome, the 5′ leader. The architecture of the 5′ leader remains controversial. Keane et al. developed nuclear magnetic resonance methods to determine the structure of a 155-nucleotide-long region of the 5′ leader that can direct viral packaging. The structure shows how the 5′ leader binds to the HIV protein that directs packaging, how unspliced dimeric genomes are selected for packaging, and how translation is suppressed when the genome dimerizes. Science , this issue p. 917

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Crossref · 10.1126/science.aaa9266

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  • Full-length Gag preferentially binds to a G//AGG loop in SL1, rather than the GGAG tetraloop in SL3.

    “the full-length Gag was shown to preferentially bind to a G//AGG loop in SL1, rather than the GGAG tetraloop in SL3”

    From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Keane et al. 2015

    The passage on NC-binding sites (section labeled Abstract; page unavailable) reports binding-site changes after mutation of the ψ GGAG loop and junction guanosines. The Gag-binding passage discusses a three-way junction, but neither passage compares full-length Gag binding to an SL1 G//AGG loop versus an SL3 GGAG loop. Searches for those loops and for full-length Gag binding found no such comparison in our copy. (LLM, conf=0.75)

    Experimental — accuracy not yet measured

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Authors
Keane, S. C., Heng, X., Lu, K., Kharytonchyk, S., Ramakrishnan, V., Carter, G., Barton, S., Hosic, A., Florwick, A., Santos, J., Bolden, N. C., McCowin, S., Case, D. A., Johnson, B. A., Salemi, M., Telesnitsky, A., & Summers, M. F.
Affiliations
  • Howard Hughes Medical Institute (US)
  • University of Michigan (US)
  • Rutgers, The State University of New Jersey (US)
  • City University of New York (US)
  • University of Florida Health (US)
Corresponding author
  • Telesnitsky, Alice
  • Summers, Michael F.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2015-05-22
Volume
348
Issue
6237
Pages
917-921
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.aaa9266
PMC
PMC4492308
OpenAlex
W2108699324
Cited by
278
References
4
Citations by year
2026: 9 · 2025: 22 · 2024: 24 · 2023: 15 · 2022: 27 · 2021: 28 · 2020: 29 · 2019: 27 · 2018: 29 · 2017: 20 · 2016: 37 · 2015: 11
FWCI
16.36
Type
journal article
Language
en
Open access
Licence
www.sciencemag.org/about/science-licenses-journal-article-reuse (vor, 366 d)
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Rutgers University Community Repository (Rutgers University) (submittedVersion)
Funders
National Institute of General Medical Sciences (R01 GM42561, P50 GM 103297)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Keywords
HIV-1, RNA packaging signal, 5' leader, RNA tertiary structure, three-way junction, translation attenuation, viral RNA structure
MeSH terms
Base Sequence, Guanosine, Molecular Sequence Data, Nucleic Acid Conformation, Peptide Chain Initiation, Translational, RNA Splicing, RNA, Viral, HIV-1, Genome, Viral, Virus Assembly, Nuclear Magnetic Resonance, Biomolecular, gag Gene Products, Human Immunodeficiency Virus

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