The TopoVIB-Like protein family is required for meiotic DNA double-strand break formation
Abstract
A partner protein for meiotic snip: Eukaryotes generate germ cells through meiotic recombination. This process initiates through breaks in genomic DNA catalyzed by the SPO11 protein. Vrielynck et al. and Robert et al. discover that SPO11, like topoisomerase VI enzymes, interacts with a partner protein (see the Perspective by Bouuaert and Keeney). This partner is required for proper meiotic recombination and is found in a wide range of eukaryotes, suggesting that it is a universal feature of the essential recombination step. Science , this issue p. 939 , 943 ; see also p. 916
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The model clarifies the Spo11 portion of the Rec102-Spo11 interface, which was not well structured in previous homology models.
“The model clarifies the Spo11 portion of this interface, which was not well structured in previous homology models (27,31).”
From Computed structures of core eukaryotic protein complexes (2021) · cites this paper as [31]
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Computed structures of core eukaryotic protein complexes
Humphreys, I. R., Pei, J., Baek, M., Krishnakumar, A., Anishchenko, I., Ovchinnikov, S., Zhang, J., Ness, T. J., Banjade, S., Bagde, S. R., et al. (2021) · Science · doi:10.1126/science.abm4805 · Cited by 542 · see citing works
Structural and functional characterization of the Spo11 core complex
Claeys Bouuaert, C., Tischfield, S. E., Pu, S., Mimitou, E. P., Arias-Palomo, E., Berger, J. M., & Keeney, S. (2021) · Nature Structural & Molecular Biology · doi:10.1038/s41594-020-00534-w · Cited by 87 · see citing works
- Authors
- Robert, T., Nore, A., Brun, C., Maffre, C., Crimi, B., Guichard, V., Bourbon, H.-M., & de Massy, B.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Centre de Biologie du Développement (FR)
- Institute of Human Genetics, UPR 1142 CNRS, University of Montpellier, 141 Rue de la Cardonille, 34396 Montpellier cedex 05, France.
- Corresponding author
- Bourbon, H.-M.
- Venue
- Science
- Published
- 2016-02-26
- Volume
- 351
- Issue
- 6276
- Pages
- 943-949
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2275186331
- Cited by
- 302
- References
- 32
- Citations by year
- 2026: 22 · 2025: 30 · 2024: 27 · 2023: 23 · 2022: 37 · 2021: 36 · 2020: 35 · 2019: 30 · 2018: 28 · 2017: 20 · 2016: 14
- FWCI
- 16.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- CNRS
- European Research Council
- European Community's Seventh Framework Programme (322788)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer therapeutics and mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- meiotic recombination, Spo11, protein complex formation, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Drosophila melanogaster, mice
- MeSH terms
- Meiotic Recombination Protein SPO11, Amino Acid Sequence, Animals, Catalysis, Chromosomal Proteins, Non-Histone, DNA Topoisomerases, Type II, DNA-Binding Proteins, Endodeoxyribonucleases, Meiosis, Molecular Sequence Data, Plant Proteins, Sequence Alignment, Conserved Sequence, Protein Structure, Tertiary, Archaeal Proteins, Protein Interaction Mapping, Saccharomyces cerevisiae Proteins, Drosophila Proteins, Recombinases, Mice, DNA Breaks, Double-Stranded
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