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Cited by 338Microtubule and mitosis dynamicsCellular Mechanics and InteractionsCardiomyopathy and Myosin Studies

Detyrosinated microtubules buckle and bear load in contracting cardiomyocytes

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Robison, Patrick, Caporizzo, Matthew A., Ahmadzadeh, Hossein, Bogush, Alexey I., Chen, Christina Yingxian, Margulies, Kenneth B., Shenoy, Vivek B., Prosser, Benjamin L.·2016·Science
DOI 10.1126/science.aaf0659PMC PMC5441927
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Abstract

A close-up view of cardiac cell mechanics: Heart cells contain a very well-organized array of cytoskeletal elements, including actin and microtubules that help them to perform their mechanical functions. Robison et al. used an advanced imaging approach to study the inner workings of mouse cardiac myocytes in real time. They observed microtubule “buckling” under contractile force in beating cardiomyocytes. This buckling was regulated by interaction with desmin and by the tubulin tyrosination state. The findings suggest a role for stable detyrosinated microtubules whose buckling under tension contributes to cardiac muscle strength. Science , this issue p. 10.1126/science.aaf0659

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  • Stable microtubules are not static; they display sliding at approximately 0.7 μm/s, active curvature induction, deformation, breaking, and occasional depolymerization.

    “We found that, although these MTs were stable, they were far from static and displayed distinct behaviors such as sliding (at ∼0.7 µm/s, Fig. 3, C and F; Video 4), active curvature induction (Fig. 3 E; Video 5), deformation (Fig. S3 A), and breaking (Fig. S3 B), consistent with earlier reports (Xu et al., 2017; Robison et al., 2016; Katrukha et al., 2017).”

    From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Robison et al., 2016

    In “MTs buckle under contractile load” (page unavailable), Robison et al. report that microtubules deform and buckle during cardiomyocyte contraction, supporting the cited comparison that stable microtubules need not be static. The Discussion (page unavailable) says they normally buckle rather than break or slide; the paper does not report sliding at ∼0.7 µm/s, active curvature induction, or occasional depolymerization. Its sliding-anchor mechanism is a model, not an observation of microtubule sliding. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Robison, P., Caporizzo, M. A., Ahmadzadeh, H., Bogush, A. I., Chen, C. Y., Margulies, K. B., Shenoy, V. B., & Prosser, B. L.
Affiliations
University of Pennsylvania (US)
Corresponding author
Prosser, Benjamin L.
ORCID
Venue
Science
Publisher
American Association for the Advancement of Science (AAAS)
Published
2016-04-22
Volume
352
Issue
6284
Pages
aaf0659-aaf0659
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
DOI
10.1126/science.aaf0659
PMC
PMC5441927
OpenAlex
W2336853363
Cited by
338
References
1
Citations by year
2026: 32 · 2025: 31 · 2024: 32 · 2023: 33 · 2022: 39 · 2021: 36 · 2020: 40 · 2019: 33 · 2018: 29 · 2017: 27 · 2016: 6
FWCI
18.29
Type
journal article
Language
en
Open access
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • National Institute for Arthritis and Musculoskeletal and Skin Diseases (NIH) (T32AR053461-09, T32HL007954)
  • National Heart, Lung, and Blood Institute (NIH) (R00-HL114879)
  • NSF Polymers Program (DMR09-07493)
  • NIH (HL089847, HL105993)
  • National Institute of Biomedical Imaging and Bioengineering (NIH) (R01EB017753)
  • NSF (DMR08-32802, CMMI-1312392)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
Keywords
microtubules, detyrosination, alpha-tubulin, cardiomyocyte contraction, sarcomere, desmin, microtubule buckling, cytoskeleton mechanics, mechanical signal transduction, cardiomyopathy
MeSH terms
Animals, Desmin, Elasticity, Heart Failure, Humans, Male, Microtubules, Models, Biological, Myocardial Contraction, Peptide Synthases, Protein Processing, Post-Translational, Sarcomeres, Tubulin, Tyrosine, Rats, Sprague-Dawley, Myocytes, Cardiac, RNA, Small Interfering, Mice, Rats

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