Cited by 293Microtubule and mitosis dynamicsNeurogenesis and neuroplasticity mechanismsGenetic and Kidney Cyst Diseases

Vasohibins/SVBP are tubulin carboxypeptidases (TCPs) that regulate neuron differentiation

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Aillaud, Chrystelle, Bosc, Christophe, Peris, Leticia, Bosson, Anouk, Heemeryck, Pierre, Van Dijk, Juliette, Le Friec, Julien, Boulan, Benoit +14 more·2017·Science

Abstract

Tubulin carboxypeptidase identity revealed: Enzymes of the α-tubulin detyrosination/tyrosination cycle create landmarks on microtubules that are essential for their multiple cellular functions and are altered in disease. Tubulin carboxypeptidases (TCPs) responsible for detyrosination have remained elusive for 40 years (see the Perspective by Akhmanova and Maiato). Aillaud et al. identified vasohibins as enzymes that perform the TCP function and found that their small interacting partner SBVP was essential for their activity. Vasohibin/SVBP complexes were involved in neuron polarization and brain cortex development. The authors also developed an inhibitor targeting this family of enzymes. Using a completely different strategy, Nieuwenhuis et al. also showed that vasohibins can remove the C-terminal tyrosine of α-tubulin. Science , this issue p. 1448 , p. 1453 ; see also p. 1381

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  • Induction of microtubule detyrosination by overexpressing VASH2-SVBP or MATCAP either did not impact the localization of GEF-H1 to microtubules or showed a slight increase in binding.

    “Induction of microtubule detyrosination (removal of the C-terminal tyrosine of α-tubulin), another modification associated with microtubule stability 31 , by overexpressing the detyrosinating enzymes VASH2-SVBP or MATCAP 56-58 , either did not impact the localization of GEF-H1 to microtubules or showed a slight increase in binding (Extended Data Fig. 4b-e).”

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Authors
Aillaud, C., Bosc, C., Peris, L., Bosson, A., Heemeryck, P., Van Dijk, J., Le Friec, J., Boulan, B., Vossier, F., Sanman, L. E., Syed, S., Amara, N., Couté, Y., Lafanechère, L., Denarier, E., Delphin, C., Pelletier, L., Humbert, S., Bogyo, M., Andrieux, A., Rogowski, K., & Moutin, M.-J.
Affiliations
  • Inserm (FR)
  • Institut de Génétique Humaine (IGH), Université Montpellier, CNRS UMR9002, 34000 Montpellier, France.
  • Grenoble Institut des Neurosciences (GIN), Université Grenoble Alpes, F-38000 Grenoble, France.
  • Stanford Medicine (US)
  • Team Regulation and Pharmacology of the Cytoskeleton, Institute for Advanced Biosciences, INSERM U 1209, CNRS UMR 5309, Université Grenoble Alpes, 38000 Grenoble, France.
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
  • Andrieux, Annie
  • Rogowski, Krzysztof
  • Moutin, Marie-Jo
Venue
Science
Published
2017-12-15
Volume
358
Issue
6369
Pages
1448-1453
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2769672674
Cited by
293
References
37
Citations by year
2026: 24 · 2025: 40 · 2024: 33 · 2023: 31 · 2022: 38 · 2021: 35 · 2020: 40 · 2019: 33 · 2018: 16 · 2017: 3
FWCI
13.72
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • HAL (submittedVersion, other-oa)
Funders
  • National Institutes of Health (EB005011)
  • Association pour la Recherche sur le Cancer (SFI20111204053, DOC20120605000, 20151203348)
  • Agence Nationale de la Recherche (ANR-10-INBS-08-01, ANR-13-JSV2-0002/TYRTUB)
  • Institut national de la santé et de la recherche médicale
  • Centre National de la Recherche Scientifique
  • Commissariat à l"Énergie Atomique et aux Énergies Alternatives
  • Université Grenoble Alpes
  • La Ligue contre le Cancer, comités Isère et Savoie
  • Fondation pour la Recherche Médicale (FDT20160435356)
  • Institut National Du Cancer (2016-165)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
vasohibin-1, vasohibin-2, microtubule dynamics, neuronal differentiation, neuronal migration, neocortex development, chemoproteomics, irreversible enzyme inhibitors
MeSH terms
Animals, Carboxypeptidases, Carrier Proteins, Cell Movement, Female, Humans, Male, Neurons, Tubulin, Tyrosine, Cell Cycle Proteins, Neocortex, Proteomics, Angiogenic Proteins, Mice, Neurogenesis, Gene Knockdown Techniques, HEK293 Cells

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