The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
Systems-level analysis of mechanisms regulating yeast metabolic flux
Open accessAbstract
Quantitation of metabolic pathway regulation: Although metabolic biochemical pathways are well understood, less is known about precisely how reaction rates or fluxes through the various enzymes are controlled. Hackett et al. developed a method to quantitate such regulatory influence in yeast. They monitored concentrations of metabolites, enzymes, and potential regulators by LC-MS/MS (liquid chromatography–tandem mass spectrometry) and isotope ratio measurements for 56 reactions, over 100 metabolites, and 370 metabolic enzymes in yeast in 25 different steady-state conditions. Bayesian analysis was used to examine the probability of regulatory interactions. Regulation of flux through the pathways was predominantly controlled by changes in the concentration of small-molecule metabolites rather than changes in enzyme abundance. The analysis also revealed previously unrecognized regulation between pathways. Science , this issue p. 432
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Metadata
- Authors
- Hackett, S. R., Zanotelli, V. R. T., Xu, W., Goya, J., Park, J. O., Perlman, D. H., Gibney, P. A., Botstein, D., Storey, J. D., & Rabinowitz, J. D.
- Venue
- Science
- Published
- 2016-10-28
- Volume
- 354
- Issue
- 6311
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2539530515
- Cited by
- 294
- References
- 71
- FWCI
- 14.75
- Type
- journal article
- Language
- en
- Open access
- green
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