The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
CRISPR-CasΦ from huge phages is a hypercompact genome editor
Open accessAbstract
Compact defense system in bacteriophages: The CRISPR-Cas system, naturally found in many prokaryotes, is widely used for genome editing. CRISPR arrays in the bacterial genome, derived from the genome of invading viruses, are used to generate a CRISPR RNA that guides the Cas enzyme to destroy repeat viral invaders. Recently, an unexpectedly compact CRISPR-Cas system was identified in huge bacteriophages. Pausch et al . show that even though this system lacks commonly found accessory proteins, it is functional. In addition to a CRISPR array, the only component of the system is an enzyme called CasF, which uses the same active site to process transcripts of the CRISPR arrays into CRISPR RNA and to destroy foreign nucleic acids. This system, which is active in human and plant cells, provides a hypercompact addition to the genome-editing toolbox. Science this issue p. 333
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RNA-Guided Human Genome Engineering via Cas9
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CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes
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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System
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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
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Self-citationNucleic acid detection with CRISPR-Cas13a/C2c2
Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia, N., Daringer, N. M., Freije, C. A., et al. (2017) · Science · doi:10.1126/science.aam9321 · Cited by 3,844
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CRISPR-Cas guides the future of genetic engineering
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Self-citationProgrammed DNA destruction by miniature CRISPR-Cas14 enzymes
Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018) · Science · doi:10.1126/science.aav4294 · Cited by 1,247
Self-citation
Cited by
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Past, present, and future of CRISPR genome editing technologies
Pacesa, M., Pelea, O., & Jinek, M. (2024) · Cell · doi:10.1016/j.cell.2024.01.042 · Cited by 609
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The CRISPR-Cas toolbox and gene editing technologies
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Metadata
- Authors
- Pausch, P., Al-Shayeb, B., Bisom-Rapp, E., Tsuchida, C. A., Li, Z., Cress, B. F., Knott, G. J., Jacobsen, S. E., Banfield, J. F., & Doudna, J. A.
- Venue
- Science
- Published
- 2020-07-17
- Volume
- 369
- Issue
- 6501
- Pages
- 333-337
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W3042599313
- Cited by
- 555
- References
- 29
- FWCI
- 22.34
- Type
- journal article
- Language
- en
- Open access
- green
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