In-cell architecture of an actively transcribing-translating expressome
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Abstract
Integrative in-cell structural biology: In bacteria, RNA polymerases can associate with ribosomes to form transcription-translation units called expressomes. Multiple models based on structural data of in vitro reconstitution assays have been proposed for how the two machineries interface with one another. Understanding this bacteria-specific coupling mechanism offers insight regarding the central dogma of molecular biology and might be leveraged for antibiotic development. O'Reilly et al. found that the NusA protein interfaces between the two complexes. The authors combined cryo–electron tomography and cross-linking mass spectrometry to produce an integrative model of the transcribing, translating expressome of Mycoplasma pneumoniae obtained entirely from in-cell data. This approach contributes to the development of in-cell structural biology. Science , this issue p. 554
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A paradigm shift in structural biology
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The Structural Dynamics of Translation
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The Ongoing Quest to Crack the Genetic Code for Protein Production
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Self-citationXlink Mapping and AnalySis (XMAS) - Smooth Integrative Modeling in ChimeraXPreprint
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Self-citationCross-linking of the endolysosomal system reveals potential flotillin structures and cargo
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Bringing the genetically minimal cell to life on a computer in 4D
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Regulation of bacterial gene expression by non-coding RNA: It is all about time!
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Structural insights into context-dependent inhibitory mechanisms of chloramphenicol in cells
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Extraribosomal Functions of Bacterial Ribosomal Proteins—An Update, 2023
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Incomplete transcripts dominate the Mycobacterium tuberculosis transcriptome
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Visualizing chaperonin function in situ by cryo-electron tomography
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Structural Heterogeneities of the Ribosome: New Frontiers and Opportunities for Cryo-EM
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Self-citationFilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis
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Revealing secrets of the enigmatic omega subunit of bacterial RNA polymerase
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Self-citationThe World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches
Gerovac, M., Vogel, J., & Smirnov, A. (2021) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2021.661448 · Cited by 21 · see citing works
Macromolecular assemblies supporting transcription-translation coupling
Webster, M. W., & Weixlbaumer, A. (2021) · Transcription · doi:10.1080/21541264.2021.1981713 · Cited by 22 · see citing works
Revealing bacterial cell biology using cryo-electron tomography
Khanna, K., & Villa, E. (2022) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2022.102419 · Cited by 22 · see citing works
- Authors
- O’Reilly, F. J., Xue, L., Graziadei, A., Sinn, L., Lenz, S., Tegunov, D., Blötz, C., Singh, N., Hagen, W. J. H., Cramer, P., Stülke, J., Mahamid, J., & Rappsilber, J.
- Affiliations
- Technische Universität Berlin (DE)
- Heidelberg University (DE)
- Max Planck Institute for Biophysical Chemistry (DE)
- University of Göttingen (DE)
- European Molecular Biology Laboratory (DE)
- Wellcome Centre for Cell Biology (GB)
- Corresponding author
- O’Reilly, Francis J.
- Xue, Liang
- Graziadei, Andrea
- Mahamid, Julia
- Rappsilber, Juri
- ORCID
- Venue
- Science
- Published
- 2020-07-31
- Volume
- 369
- Issue
- 6503
- Pages
- 554-557
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC7115962
- OpenAlex
- W3046695191
- Cited by
- 284
- References
- 96
- Citations by year
- 2026: 29 · 2025: 28 · 2024: 48 · 2023: 52 · 2022: 44 · 2021: 61 · 2020: 21 · 2019: 1
- FWCI
- 14.42
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Science (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, other-oa)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Wellcome (103139)
- European Research Council (760067, 693023)
- Deutsche Forschungsgemeinschaft (426290502, EXC 2067/1-390729940, EXC 2008 – 390540038)
- Bundesministerium für Bildung und Forschung (FKZ 031L0012)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
- Keywords
- transcription-translation coupling, RNA polymerase, ribosome, Mycoplasma pneumoniae, cryo-electron tomography, cross-linking mass spectrometry, antibiotic development
- MeSH terms
- Bacterial Proteins, Humans, Mycoplasma pneumoniae, Peptide Chain Elongation, Translational, Peptide Elongation Factors, Ribosomes, DNA-Directed RNA Polymerases, Transcription, Genetic, Genome, Bacterial, Transcriptome, Protein Interaction Maps
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