Cited by 284RNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyAdvanced Electron Microscopy Techniques and Applications

In-cell architecture of an actively transcribing-translating expressome

Open access
O’Reilly, Francis J., Xue, Liang, Graziadei, Andrea, Sinn, Ludwig, Lenz, Swantje, Tegunov, Dimitry, Blötz, Cedric, Singh, Neil +5 more·2020·Science

Published version of a preprint

Abstract

Integrative in-cell structural biology: In bacteria, RNA polymerases can associate with ribosomes to form transcription-translation units called expressomes. Multiple models based on structural data of in vitro reconstitution assays have been proposed for how the two machineries interface with one another. Understanding this bacteria-specific coupling mechanism offers insight regarding the central dogma of molecular biology and might be leveraged for antibiotic development. O'Reilly et al. found that the NusA protein interfaces between the two complexes. The authors combined cryo–electron tomography and cross-linking mass spectrometry to produce an integrative model of the transcribing, translating expressome of Mycoplasma pneumoniae obtained entirely from in-cell data. This approach contributes to the development of in-cell structural biology. Science , this issue p. 554

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Authors
O’Reilly, F. J., Xue, L., Graziadei, A., Sinn, L., Lenz, S., Tegunov, D., Blötz, C., Singh, N., Hagen, W. J. H., Cramer, P., Stülke, J., Mahamid, J., & Rappsilber, J.
Affiliations
  • Technische Universität Berlin (DE)
  • Heidelberg University (DE)
  • Max Planck Institute for Biophysical Chemistry (DE)
  • University of Göttingen (DE)
  • European Molecular Biology Laboratory (DE)
  • Wellcome Centre for Cell Biology (GB)
Corresponding author
  • O’Reilly, Francis J.
  • Xue, Liang
  • Graziadei, Andrea
  • Mahamid, Julia
  • Rappsilber, Juri
Venue
Science
Published
2020-07-31
Volume
369
Issue
6503
Pages
554-557
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W3046695191
Cited by
284
References
96
Citations by year
2026: 29 · 2025: 28 · 2024: 48 · 2023: 52 · 2022: 44 · 2021: 61 · 2020: 21 · 2019: 1
FWCI
14.42
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Science (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, other-oa)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Funders
  • Wellcome (103139)
  • European Research Council (760067, 693023)
  • Deutsche Forschungsgemeinschaft (426290502, EXC 2067/1-390729940, EXC 2008 – 390540038)
  • Bundesministerium für Bildung und Forschung (FKZ 031L0012)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
Keywords
transcription-translation coupling, RNA polymerase, ribosome, Mycoplasma pneumoniae, cryo-electron tomography, cross-linking mass spectrometry, antibiotic development
MeSH terms
Bacterial Proteins, Humans, Mycoplasma pneumoniae, Peptide Chain Elongation, Translational, Peptide Elongation Factors, Ribosomes, DNA-Directed RNA Polymerases, Transcription, Genetic, Genome, Bacterial, Transcriptome, Protein Interaction Maps

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