Single-cell Stereo-seq reveals induced progenitor cells involved in axolotl brain regeneration
Abstract
The molecular mechanism underlying brain regeneration in vertebrates remains elusive. We performed spatial enhanced resolution omics sequencing (Stereo-seq) to capture spatially resolved single-cell transcriptomes of axolotl telencephalon sections during development and regeneration. Annotated cell types exhibited distinct spatial distribution, molecular features, and functions. We identified an injury-induced ependymoglial cell cluster at the wound site as a progenitor cell population for the potential replenishment of lost neurons, through a cell state transition process resembling neurogenesis during development. Transcriptome comparisons indicated that these induced cells may originate from local resident ependymoglial cells. We further uncovered spatially defined neurons at the lesion site that may regress to an immature neuron–like state. Our work establishes spatial transcriptome profiles of an anamniote tetrapod brain and decodes potential neurogenesis from ependymoglial cells for development and regeneration, thus providing mechanistic insights into vertebrate brain regeneration.
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Previously, spatial transcriptomics technology has been utilized to create spatiotemporal maps of various life stages, including mouse brain, regenerating axolotl telencephalons, aging ovary, and brain.
“Previously, this technology has been utilized to create spatiotemporal maps of various life stages, including mouse brain, regenerating axolotl telencephalons, aging ovary, and brain.21”
From Spatial transcriptomic landscape unveils immunoglobin-associated senescence as a hallmark of aging (2024) · cites this paper as [37]
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- Authors
- Wei, X., Fu, S., Li, H., Liu, Y., Wang, S., Feng, W., Yang, Y., Liu, X., Zeng, Y.-Y., Cheng, M., Lai, Y., Qiu, X., Wu, L., Zhang, N., Jiang, Y., Xu, J., Su, X., Peng, C., Han, L., Lou, W. P.-K., Liu, C., Yuan, Y., Ma, K., Yang, T., Pan, X., Gao, S., Chen, A., Esteban, M. A., Yang, H., Wang, J., Fan, G., Liu, L., Chen, L., Xu, X., Fei, J.-F., & Gu, Y.
- Affiliations
- BGI Group (China) (CN)
- South China Normal University (CN)
- Zhengzhou University (CN)
- BGI-Qingdao, Qingdao 266555, China.
- Chinese Academy of Sciences (CN)
- Howard Hughes Medical Institute (US)
- Guangdong Academy of Medical Sciences (CN)
- Wuhan University (CN)
- Corresponding author
- Wei, Xiaoyu
- Fu, Sulei
- Li, Hanbo
- Liu, Yang
- Wang, Shuai
- Feng, Weimin
- Yang, Yunzhi
- Chen, Liang
- Xu, Xun
- Fei, Ji-Feng
- Gu, Ying
- ORCID
- Wei, Xiaoyu
- Fu, Sulei
- Li, Hanbo
- Liu, Yang
- Wang, Shuai
- Feng, Weimin
- Yang, Yunzhi
- Lai, Yiwei
- Qiu, Xiaojie
- Wu, Liang
- Zhang, Nannan
- Jiang, Yujia
- Xu, Jiangshan
- Su, Xiaoshan
- Peng, Cheng
- Han, Lei
- Lou, Wilson Pak-Kin
- Liu, Chuanyu
- Yuan, Yue
- Ma, Kailong
- Yang, Tao
- Pan, Xiangyu
- Gao, Shang
- Chen, Ao
- Esteban, Miguel A.
- Yang, Huanming
- Wang, Jian
- Fan, Guangyi
- Liu, Longqi
- Chen, Liang
- Xu, Xun
- Fei, Ji-Feng
- Gu, Ying
- Venue
- Science
- Published
- 2022-09-02
- Volume
- 377
- Issue
- 6610
- Pages
- eabp9444-eabp9444
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W4294042823
- Cited by
- 311
- References
- 9
- Citations by year
- 2026: 69 · 2025: 93 · 2024: 80 · 2023: 58 · 2022: 11
- FWCI
- 23.01
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Single-cell and spatial transcriptomics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- axolotl, brain regeneration, telencephalon, spatial transcriptomics, cell state transitions, neurogenesis
- MeSH terms
- Brain Regeneration, Ambystoma mexicanum, Animals, Telencephalon, Neural Stem Cells, Single-Cell Analysis, Transcriptome
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