Direct observation of motor protein stepping in living cells using MINFLUX
Open accessPublished version of a preprint
Abstract
Dynamic measurements of molecular machines can provide invaluable insights into their mechanism, but these measurements have been challenging in living cells. Here, we developed live-cell tracking of single fluorophores with nanometer spatial and millisecond temporal resolution in two and three dimensions using the recently introduced super-resolution technique MINFLUX. Using this approach, we resolved the precise stepping motion of the motor protein kinesin-1 as it walked on microtubules in living cells. Nanoscopic tracking of motors walking on the microtubules of fixed cells also enabled us to resolve the architecture of the microtubule cytoskeleton with protofilament resolution.
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Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · see citing works
ColabFold - Making protein folding accessible to all
Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2021) · doi:10.1101/2021.08.15.456425 · Cited by 9,391 · see citing works
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Kinesin׳s tail domain is an inhibitory regulator of the motor domain
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Self-citation‘MINFLUX monitors rapid molecular jumps with superior spatiotemporal resolution’
Eilers, Y., Ta, H., Gwosch, K. C., Balzarotti, F., & Hell, S. W. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1801672115 · Cited by 188 · see citing works
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Mechanism and regulation of kinesin motors.
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- Authors
- Deguchi, T., Iwanski, M. K., Schentarra, E.-M., Heidebrecht, C., Schmidt, L., Heck, J., Weihs, T., Schnorrenberg, S., Hoess, P., Liu, S., Chevyreva, V., Noh, K.-M., Kapitein, L. C., & Ries, J.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Utrecht University (NL)
- Heidelberg University (DE)
- Abberior Instruments GmbH, Göttingen, Germany.
- University of New Mexico (US)
- IFOM (IT)
- Corresponding author
- Ries, Jonas
- ORCID
- Venue
- Science
- Published
- 2023-03-10
- Volume
- 379
- Issue
- 6636
- Pages
- 1010-1015
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- PMC
- PMC7614483
- OpenAlex
- W4323811077
- Cited by
- 167
- References
- 6
- Citations by year
- 2026: 30 · 2025: 51 · 2024: 51 · 2023: 34 · 2022: 1
- FWCI
- 20.10
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by
- Locations
- Science (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- European Molecular Biology Laboratory (847543, EMBL001, EIPOD4)
- Horizon 2020 Framework Programme (847543, CoG-724489)
- H2020 European Research Council (819219)
- H2020 Marie Skłodowska-Curie Actions
- HORIZON EUROPE Marie Sklodowska-Curie Actions (847543, H2020, 945405)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Physical Sciences › Physics and Astronomy › Atomic and Molecular Physics, and Optics › Force Microscopy Techniques and Applications
- Keywords
- MINFLUX, kinesin-1, microtubules, super-resolution microscopy, molecular machines, microtubule protofilaments
- MeSH terms
- Cells, Fluorescent Dyes, Humans, Microscopy, Fluorescence, Microtubules, Motion, Kinesins
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