Cited by 75Advanced Fluorescence Microscopy TechniquesCell Image Analysis TechniquesImage Processing Techniques and Applications

Live-cell imaging powered by computation

Open access
Shroff, Hari, Testa, Ilaria, Jug, Florian, Manley, Suliana·2024·Nature Reviews Molecular Cell Biology

Abstract

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. The prevailing challenge in live-cell fluorescence microscopy is capturing intra-cellular dynamics while preserving cell viability. Computational methods can help to address this challenge and are now shifting the boundaries of what is possible to capture in living systems. In this Review, we discuss these computational methods focusing on artificial intelligence-based approaches that can be layered on top of commonly used existing microscopies as well as hybrid methods that integrate computation and microscope hardware. We specifically discuss how computational approaches can improve the signal-to-noise ratio, spatial resolution, temporal resolution and multi-colour capacity of live-cell imaging.

Integrity signals

Registry

Crossref · 10.1038/s41580-024-00702-6

Cites

133 of 133 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Live-cell imaging plays a crucial role in biological experiments, offering insights into cellular activities.

    “Beyond clinical imaging, live-cell imaging plays a crucial role in biological experiments, offering insights into cellular activities [37].”

Cites

Flagged first, then by citations

3 of the 136 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Shroff, H., Testa, I., Jug, F., & Manley, S.
Affiliations
  • Howard Hughes Medical Institute (US)
  • Science for Life Laboratory (SE)
  • Human Technopole (IT)
  • École Polytechnique Fédérale de Lausanne (CH)
Corresponding author
Manley, Suliana
Venue
Nature Reviews Molecular Cell Biology
Published
2024-02-20
Volume
25
Issue
6
Pages
443-463
ISSN
  • 1471-0072 (ISSN-L)
  • 1471-0080
OpenAlex
W4391971327
Cited by
75
References
136
Citations by year
2026: 27 · 2025: 35 · 2024: 13
FWCI
4.07
Type
journal article
Language
en
Open access
Locations
  • Nature Reviews Molecular Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
  • Physical Sciences › Engineering › Media Technology › Image Processing Techniques and Applications
Keywords
live-cell imaging, computational microscopy, machine learning, artificial intelligence, fluorescence microscopy, signal-to-noise ratio, spatial resolution, temporal resolution, cell viability
MeSH terms
Animals, Artificial Intelligence, Cell Survival, Humans, Image Processing, Computer-Assisted, Microscopy, Fluorescence, Signal-To-Noise Ratio

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review