Cited by 253Enzyme Catalysis and ImmobilizationRadical Photochemical ReactionsAsymmetric Synthesis and Catalysis

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Stereoselective amino acid synthesis by synergistic photoredox-pyridoxal radical biocatalysis

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Cheng, Lei, Li, Dian, Mai, Binh Khanh, Bo, Zhiyu, Cheng, Lida, Liu, Peng, Yang, Yang·2023·Science

DOI 10.1126/science.adg2420PMC PMC10444520

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Abstract

Developing synthetically useful enzymatic reactions that are not known in biochemistry and organic chemistry is an important challenge in biocatalysis. Through the synergistic merger of photoredox catalysis and pyridoxal 5′-phosphate (PLP) biocatalysis, we developed a pyridoxal radical biocatalysis approach to prepare valuable noncanonical amino acids, including those bearing a stereochemical dyad or triad, without the need for protecting groups. Using engineered PLP enzymes, either enantiomeric product could be produced in a biocatalyst-controlled fashion. Synergistic photoredox - pyridoxal radical biocatalysis represents a powerful platform with which to discover previously unknown catalytic reactions and to tame radical intermediates for asymmetric catalysis.

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Authors: Cheng, L., Li, D., Mai, B. K., Bo, Z., Cheng, L., Liu, P., & Yang, Y.
Affiliations: University of California, Santa Barbara (US)
University of Pittsburgh (US)
Corresponding author: Liu, Peng
Yang, Yang
ORCID: Cheng, Lei
Li, Dian
Mai, Binh Khanh
Bo, Zhiyu
Cheng, Lida
Liu, Peng
Yang, Yang
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 2023-07-28
Volume: 381
Issue: 6656
Pages: 444-451
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.adg2420
PMC: PMC10444520
OpenAlex: W4385298585
Cited by: 253
References: 123
Citations by year: 2026: 80 · 2025: 105 · 2024: 57 · 2023: 11
FWCI: 22.35
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Science (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Science Foundation (1933487)
University of Pittsburgh
Herman Frasch Foundation for Chemical Research (947-HF22)
Xiamen University
National Institutes of Health
University of California, Santa Barbara
National Institute of General Medical Sciences (R35GM147387, R35GM128779)
Division of Materials Research (. DMR-1933487, 1933487)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
Physical Sciences › Chemistry › Organic Chemistry › Radical Photochemical Reactions
Physical Sciences › Chemistry › Organic Chemistry › Asymmetric Synthesis and Catalysis
Keywords: unnatural amino acids, photoredox catalysis, asymmetric catalysis, radical intermediates, engineered enzymes
MeSH terms: Amino Acids, Pyridoxal Phosphate, Stereoisomerism, Biocatalysis

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Stereoselective amino acid synthesis by synergistic photoredox-pyridoxal radical biocatalysis

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